Capillary isotachophoresis with fiber-optic Raman spectroscopic detection. Performance and application to ribonucleotides.
Capillary isotachophoresis with fiber-optic Raman spectroscopic detection. Performance and application to ribonucleotides.
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毛细管等速电泳与光纤拉曼光谱检测。
DOI：
10.1016/s0021-9673(97)01302-2
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Morris,MD
中科院分区：
文献类型：
--
作者：
Walker3rd,PA;Morris,MD
采用光纤拉曼探针和显微镜物镜，对毛细管等速电泳分离的5′-单磷酸腺苷、5′-单磷酸胞苷、5′-单磷酸鸟苷和5′-单磷酸尿苷进行了在线正常拉曼光谱分析。系统采用多模光纤，测量长度为40 μm的毛细管。光纤耦合便于使用未经修改的光谱仪和传统的毛细管安装系统。拉曼光谱以2W 532 nm NdYVO4激光器为激发源，每秒收集1个光谱。即使在初始浓度为2·10−5M时，也能获得良好的信噪比。
A fiber-optic Raman probe fitted with a microscope objective was used to obtain on-line normal Raman spectra of adenosine 5′-monophosphate, cytidine 5′-monophosphate, guanosine 5′-monophosphate and uridine 5′-monophosphate separated by capillary isotachophoresis. With multimode optical fiber, the system interrograted a 40-μm length of capillary. Fiber-optic coupling facilitated use of an unmodified spectrograph and conventional capillary mounting systems. Raman spectra were excited with a 2W 532 nm NdYVO4, laser as the excitation source, with collection of 1 spectrum per second. Even at 2·10−5M initial concentration, Raman spectra were obtained at a good signal-to-noise ratio.