SULOFENUR CYTOTOXICITY AND CHANGES IN CYTOSOLIC CALCIUM AND MITOCHONDRIAL-MEMBRANE POTENTIAL IN HUMAN COLON ADENOCARCINOMA CELL-LINES
SULOFENUR CYTOTOXICITY AND CHANGES IN CYTOSOLIC CALCIUM AND MITOCHONDRIAL-MEMBRANE POTENTIAL IN HUMAN COLON ADENOCARCINOMA CELL-LINES
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DOI：
10.1016/0304-3835(94)03607-k
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发表时间：
1995-01-06
期刊：
影响因子：
9.7
通讯作者：
TRUMP, BF
中科院分区：
文献类型：
--
作者：
PHELPS, PC;JAIN, PT;TRUMP, BF
结肠腺癌细胞系的sugammur处理（12.5 μ M-1 mM）导致剂量和时间依赖性的细胞杀伤，LYc（5）细胞比GC（3）/c（1）细胞存活时间长。每种浓度均导致两种细胞系的胞质钙[Ca 2 +]（i）升高。在较低剂量下，LYc（5）细胞的升高延迟，GC（3）/c（1）细胞在无Ca 2 + HBSS中1 mM处理后，[Ca 2 +]（i）未显示升高。GC（3）/c（1）细胞经羰腈间氯苯腙处理后，线粒体上的罗丹明123荧光迅速消失，经1 mM硫酰氯处理后，荧光逐渐消失。处理后，细胞变圆，形成水泡，细胞死亡。结果表明，[Ca ~（2+）]（i）升高在硫酸钠的细胞毒性中起重要作用。
Sulofenur treatment (12.5 mu M-1 mM) of colon adenocarcinoma cell lines resulted in dose- and time-dependent cell killing, LYc(5) cells were viable longer than GC(3)/c(1) cells. Each concentration resulted in elevation of cytosolic calcium [Ca2+](i)) for both cell lines. At lower doses, elevation was delayed for LYc(5) cells, GC(3)/c(1) cells after 1 mM treatment in Ca2+-free HBSS showed no rise of [Ca2+](i). GC(3)/c(1) cells after carbonyl cyanide-m-chlorophenylhydrazone rapidly lost rhodamine 123 fluorescence from mitochondria; after 1 mM sulofenur, fluorescence faded slowly. Following treatment, cells became rounded, blebs formed and the cells died. Results suggest that elevated [Ca2+](i) plays an important role in sulofenur cytotoxicity.