Signal transducer and activator of transcription 5B (STAT5B) modulates adipocyte differentiation via MOF
Signal transducer and activator of transcription 5B (STAT5B) modulates adipocyte differentiation via MOF
复制标题
信号转导器和转录激活剂 5B (STAT5B) 通过 MOF 调节脂肪细胞分化
DOI：
10.1016/j.cellsig.2015.09.010
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发表时间：
2015-12-01
影响因子：
4.8
通讯作者：
Wang, Xiangdong
中科院分区：
文献类型：
--
作者：
Gao, Peng;Zhang, Yuchao;Wang, Xiangdong
The role and mechanism of signal transducer and activator of transcription 5B (STAT5B) in adipogenesis remain unclear. In this study, our data showed that Males absent on the first (MOF) protein expression was increased during 3 T3-L1 preadipocytes differentiation accompanied with STAT5B expression increasing. Over-expression STAT5B enhanced MOP promoter trans-activation in HeLa cells. Mutagenesis assay and ChIP analysis exhibited that STAT5B was able to bind MOF promoter. Knocking-down STAT5B in 3 T3-L1 preadipocytes led to decreased expression of MOF, but resulted in increased expression of peroxisome proliferator-activated receptor gamma (PPAR gamma), CCAAT/enhancer-binding protein alpha (C/EBP alpha) and fatty acid-binding protein 4 (Fabp4), which were important factors or enzymes for adipogenesis. We also found that knocking-down MOF in 3 T3-L1 preadipocytes resulted in increased expression of PPAR gamma, C/EBP alpha and Fabp4, which was in the same trend as STAT5B knocking-down. Over-expression MOF resulted in reduced promoter trans-activation activity of C/EBP alpha. These results suggest that STAT5B and MOF work as negative regulators in adipogenesis, and STAT5B modulates preadipocytes differentiation partially by regulating MOF expression. (C) 2015 The Authors. Published by Elsevier Inc.
