Detection of sequences homologous to human retroviral DNA in multiple sclerosis by gene amplification.
Detection of sequences homologous to human retroviral DNA in multiple sclerosis by gene amplification.
复制标题
通过基因扩增检测多发性硬化症中与人逆转录病毒 DNA 同源的序列。
DOI：
10.1073/pnas.86.8.2878
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发表时间：
1989
影响因子：
11.1
通讯作者：
Poiesz,BJ
中科院分区：
文献类型：
--
作者：
Greenberg,SJ;Ehrlich,GD;Abbott,MA;Hurwitz,BJ;Waldmann,TA;Poiesz,BJ
对21例慢性进展型多发性硬化患者进行了与人类逆转录病毒同源的DNA序列检查。采用体外酶基因扩增技术和聚合酶链反应分析外周血单个核细胞基因组DNA中是否存在与人T细胞白血病/淋巴瘤病毒I型（HTLV-I）长末端重复序列、3' gag、pol和env结构域同源的序列。在这些患者中的6例（29%）的扩增DNA中进行了同源pol序列的阳性鉴定。这6名患者中有3名（14%）的env区域检测结果也呈阳性，但其他区域检测结果均为阴性。与此相反，从35个正常人的样本研究时，没有一个是阳性的扩增和测试时，用相同的引物和探针。从对照组和成人T细胞白血病或热带痉挛性下肢轻瘫患者获得的模式的比较表明，所确定的DNA序列是外源性的人类基因组，并可能对应于人类逆转录病毒物种。这些数据支持在一些多发性硬化症患者中检测到人类逆转录病毒药物。
Twenty-one patients with multiple sclerosis, chronic progressive type, were examined for DNA sequences homologous to a human retrovirus. Genomic DNA from peripheral blood mononuclear cells was analyzed for the presence of homologous sequences to the human T-cell leukemia/lymphoma virus type I (HTLV-I) long terminal repeat, 3' gag, pol, and env domains by the enzymatic in vitro gene amplification technique, polymerase chain reaction. Positive identification of homologous pol sequences was made in the amplified DNA from six of these patients (29%). Three of these six patients (14%) also tested positive for the env region, but not for the other regions tested. In contrast, none of the samples from 35 normal individuals studied was positive when amplified and tested with the same primers and probes. Comparison of patterns obtained from controls and from patients with adult T-cell leukemia or tropical spastic paraparesis suggests that the DNA sequences identified are exogenous to the human genome and may correspond to a human retroviral species. The data support the detection of a human retroviral agent in some patients with multiple sclerosis.