Immunohistochemical localization of cardiodilatin in myoendocrine cells of the cardiac atria
Immunohistochemical localization of cardiodilatin in myoendocrine cells of the cardiac atria
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心房肌内分泌细胞中心肌扩张素的免疫组织化学定位
DOI：
10.1007/bf00318996
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
W. Forssmann
中科院分区：
文献类型：
--
作者：
J. Metz;V. Mutt;W. Forssmann
在家兔中培养了针对心舒张素合成n端片段的区域特异性抗体，并用于几种动物心房肌内分泌细胞中这种新肽激素的免疫组织化学检测。过氧化物酶-抗过氧化物酶（PAP）和异硫氰酸荧光素（FITC）免疫组化方法在组织内获得相同的心舒张素免疫反应性（CDD-IR）结果。除猪右心房附件外，在猪心脏左心房以及狗、猫等其他物种中也检测到具有CDD-IR的肌内分泌细胞。特异分泌颗粒中免疫反应性的确切定位主要与位于耳肌内分泌细胞核两极的高尔基区有关。电镜观察结果证实，心肌舒张素以分泌颗粒形式存在。这种激素最有可能在肌内分泌细胞中合成和释放，发挥其重要的心血管作用。
SummaryRegion-specific antibodies against synthetic N-terminal fragments of cardiodilatin (CDD) were raised in rabbits and used for the immunohistochemical detection of this new peptide hormone in the myoendocrine cells within the cardiac atria of several species. The peroxidase-antiperoxidase (PAP) and fluorescein isothiocyanate (FITC) immunohistochemical methods gave identical results of cardiodilatin-immunoreactivity (CDD-IR) within the tissue. In addition to the porcine right atrial appendage, myoendocrine cells with CDD-IR were also detected in the left atrium of porcine heart, as well as in other species such as dog and cat. The exact localization of the immunoreactivity in specific secretory granules was mostly related to the Golgi-area which is located on both nuclear poles of auricular myoendocrine cells. The results confirm that cardiodilatin is stored in secretory granules observed through electron microscopical means. This hormone is most likely synthesized and released in myoendocrine cells, exerting its important cardiovascular effects.