Comparative study using rabbit-derived polyclonal, mouse-derived monoclonal, and rabbit-derived monoclonal antibodies for KIT immunostaining in GIST and other tumors
Comparative study using rabbit-derived polyclonal, mouse-derived monoclonal, and rabbit-derived monoclonal antibodies for KIT immunostaining in GIST and other tumors
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DOI：
10.1111/j.1440-1827.2007.02081.x
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发表时间：
2007-04-01
影响因子：
2.2
通讯作者：
Nakajima, Takashi
中科院分区：
文献类型：
--
作者：
Saito, Masako;Sakurai, Shinji;Nakajima, Takashi
为了寻找更好的KIT免疫染色条件，比较了5种KIT抗体，包括一种广泛使用的多克隆抗体(PAb)A4502，三种鼠源性单抗(MMA；T595，1DC3，K45)和一种新开发的兔源性单抗(RabMA；Y145)。23例胃肠道间质瘤(GIST)，包括5例Kit弱或阴性的GIST，6例尤文/恶性原始神经外胚叶肿瘤，6例恶性黑色素瘤，2例神经母细胞瘤，6例精原细胞瘤，7例胸腺癌和7例肺小细胞癌为Kit表达肿瘤，4例平滑肌瘤，6例平滑肌肉瘤，5例胃神经鞘瘤，4例孤立纤维瘤，1例炎性纤维样息肉和6例硬纤维样瘤为Kit非表达肿瘤。在KIT表达的肿瘤中，RabMA Y145的阳性率与Pab A4502几乎相同，而3种MMA的阳性率低于Pab A4502。MMA T595对肥大细胞呈阳性反应，而对Cajal间质细胞和部分GIST细胞呈阴性反应。在KIT非表达的肿瘤中没有一例Y145阳性，而一些平滑肌肉瘤和韧带样瘤A4502呈阳性。目前，PAb A4502或RabMA Y145似乎适合于福尔马林固定的石蜡包埋肿瘤标本的KIT免疫染色，特别是在GIST与其他间叶性肿瘤的鉴别诊断中。
To find a better condition for KIT immunostaining, five antibodies against KIT were compared, including a widely used polyclonal antibody (pAb) A4502, three mouse-derived mAb (MMA; T595, 1DC3, K45), and a newly developed rabbit-derived mAb (RabMA; Y145). Twenty-three gastrointestinal stromal tumors (GIST) were stained, including five KIT-weak or -negative GIST with PDGFRA gene mutations from a previous report, six Ewing/malignant primitive peripheral neuroectodermal tumor, six malignant melanomas, two neuroblastomas, six seminomas, seven thymic carcinoma and seven small cell carcinomas of the lung as KIT-expressing tumors, and four leiomyomas, six leiomyosarcomas, five gastric schwannomas, four solitary fibrous tumors, one inflammatory fibroid polyp and six desmoid tumors as KIT-non-expressing tumors. The positive rates of RabMA Y145 in KIT-expressing tumors were almost equal to pAb A4502, whereas those of three MMA had lower rates. MMA T595 was positive for mast cells, but negative for interstitial cells of Cajal and some GIST. None of the KIT-non-expressing tumors was positive for Y145, whereas some leiomyosarcomas and desmoid tumors were positive for A4502. At present, pAb A4502 or RabMA Y145 seems to be suitable for KIT immunostaining in formalin-fixed paraffin-embedded tumor specimens, especially in the differential diagnosis of GIST from other mesenchymal tumors.