Mapping long-range chromatin organization within the chicken α-globin gene domain using oligonucleotide DNA arrays
Mapping long-range chromatin organization within the chicken α-globin gene domain using oligonucleotide DNA arrays
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DOI：
10.1016/j.ygeno.2004.09.008
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发表时间：
2005-01-01
期刊：
影响因子：
4.4
通讯作者：
Vassetzky, YS
中科院分区：
文献类型：
--
作者：
Ioudinkova, E;Petrov, A;Vassetzky, YS
我们利用DNA微阵列分析了鸡α-珠蛋白基因结构域的组织结构，发现了两个新的染色质环连接区域。我们发现了一个40kb的环状结构域，它包括红系起源的细胞中的所有a-珠蛋白基因。其中一个结构域边界与一个强大的MAR元件和早些时候在a-珠蛋白基因结构域上游发现的一段增强子阻断元件几乎完全相同。在淋巴样细胞系DT40中发现了不同的结构域结构。我们建议使用DNA阵列技术来定位定义染色体环域边界的核基质附着位置。DNA阵列技术允许将大量的DNA序列固定在玻璃或尼龙基质上。在与包含特定DNA区域的DNA芯片的杂交中，通过使用染色质环附着区池作为探针，这可能被证明对定位人类基因组中的染色质环位置是有用的。(C)2004 Elsevier Inc.保留所有权利。
We have analyzed the organization of the chicken a-globin gene domain using DNA miniarrays and have found two novel chromatin loop attachment regions. We have found a 40-kb loop domain that includes all the a-globin genes in cells of erythroid origin. One of the domain borders colocalizes almost exactly with a strong MAR element and with a block of enhancer-blocking elements found earlier at the upstream end of the a-globin gene domain. The domain structure was found to be different in a lymphoid cell line DT40. We propose to use the technique of DNA arrays to map the nuclear matrix attachment sites that define the borders of chromosome loop domains. The technique of DNA arrays permits a large number of DNA sequences to be immobilized on a glass or nylon matrix. This may prove useful for mapping chromatin loop positions within the human genome by using a pool of chromatin loop attachment regions as a probe in a hybridization with a DNA chip containing a specific DNA region. (C) 2004 Elsevier Inc. All rights reserved.