TARGETED EXPRESSION OF TETANUS TOXIN LIGHT-CHAIN IN DROSOPHILA SPECIFICALLY ELIMINATES SYNAPTIC TRANSMISSION AND CAUSES BEHAVIORAL DEFECTS
TARGETED EXPRESSION OF TETANUS TOXIN LIGHT-CHAIN IN DROSOPHILA SPECIFICALLY ELIMINATES SYNAPTIC TRANSMISSION AND CAUSES BEHAVIORAL DEFECTS
复制标题
DOI：
10.1016/0896-6273(95)90290-2
复制
发表时间：
1995-02-01
期刊：
影响因子：
16.2
通讯作者：
OKANE, CJ
中科院分区：
文献类型：
--
作者：
SWEENEY, ST;BROADIE, K;OKANE, CJ
破伤风毒素能裂解突触小泡蛋白突触短缩蛋白，随之而来的神经递质胞吐功能的丧失使突触短缩蛋白参与了这一过程。为了进一步研究遗传上容易控制的生物体中的突触功能，并开发出一种用于行为研究的禁用神经元交流的工具，我们在果蝇中表达了一种编码破伤风毒素轻链的基因。在胚胎神经元中表达毒素可以消除可检测到的突触短缩蛋白，并消除诱发的、但不是自发的突触小泡释放。没有检测到其他发育或形态缺陷。相应地，破伤风毒素在体外只切割突触素(n-syb)，而不是普遍表达的syb蛋白。毒素的靶向表达会产生特定的行为缺陷；在一种情况下，嗅觉逃逸反应会减少。
Tetanus toxin cleaves the synaptic vesicle protein synaptobrevin, and the ensuing loss of neurotransmitter exocytosis has implicated synaptobrevin in this process. To further the study of synaptic function in a genetically tractable organism and to generate a tool to disable neuronal communication for behavioural studies, we have expressed a gene encoding tetanus toxin light chain in Drosophila. Toxin expression in embryonic neurons removes detectable synaptobrevin and eliminates evoked, but not spontaneous, synaptic vesicle release. No other developmental or morphological defects are detected. Correspondingly, only synaptobrevin (n-syb), but not the ubiquitously expressed syb protein, is cleaved by tetanus toxin in vitro. Targeted expression of toxin can produce specific behavioral defects; in one case, the olfactory escape response is reduced.