THE EFFECTS OF ALPHA-NEUROTOXIN AND BETA-NEUROTOXIN FROM THE VENOMS OF VARIOUS SNAKES ON TRANSMISSION IN AUTONOMIC GANGLIA
THE EFFECTS OF ALPHA-NEUROTOXIN AND BETA-NEUROTOXIN FROM THE VENOMS OF VARIOUS SNAKES ON TRANSMISSION IN AUTONOMIC GANGLIA
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DOI：
10.1016/0006-8993(81)90070-6
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发表时间：
1981-01-01
期刊：
影响因子：
2.9
通讯作者：
ZIGMOND, RE
中科院分区：
文献类型：
--
作者：
CHIAPPINELLI, VA;COHEN, JB;ZIGMOND, RE
某些市售的α-银环蛇毒素在10 μ g/ml（1.2 μ M）浓度下阻断鸽子和鸡睫状神经节的睫状和脉络膜神经元中的传递。通过与100 μ M筒箭毒碱预孵育来拮抗该阻断。进一步的证据表明，这种阻断是由突触后作用产生的，正如人们所预期的α-突触后作用。神经毒素，是发现：暴露于该毒素可防止神经节细胞的去极化，该去极化通常见于对胆碱能激动剂卡巴胆碱的反应;并且该毒素的阻断活性可通过用从含有过量α-氨基丁酸的电鳐电器官纯化的膜处理而去除。神经毒素结合位点。[125 I] α-的高亲和性结合位点银环蛇毒素在鸡睫状神经节中具有特征性。因为它同样被许多α-在阻断传播的银环蛇毒素和不阻断传播的银环蛇毒素之间，该位点似乎不参与阻断传播。α-眼镜蛇毒素（来自Naja naja siamensis），α-神经毒素S. III（来自Laticauda semifasciata）和某些批次的α-银环蛇毒素在10 μ g/ml（1.2 μ M）的浓度下在鸽子睫状神经节的睫状神经元中产生部分阻断传递，但对脉络膜神经元中的传递没有影响。另外两个α-神经毒素L.在该浓度下，半带纹藻毒素A和erabutoxin B对任一细胞群中的传播均无影响。没有一个α-测试的神经毒素在浓度高达100 μ g/ml（12 μ M）时对大鼠上级颈神经节或大鼠盆神经节中的传递具有任何作用。胶原酶处理这些神经节以增加毒素对神经节细胞的访问并没有改变这些阴性结果。β-银环蛇毒素（0.5 μ g/ml，0.02 μ M）在禽类睫状神经节和大鼠上级颈神经节中产生复杂的传递阻断。与α的作用不同，银环蛇毒素，通过β-环己内酯阻断睫状神经节传递，银环蛇毒素是不可逆的，并且不能通过筒箭毒碱预处理来预防。
Certain commercially available lots of .alpha.-bungarotoxin block transmission in ciliary and choroid neurons of both pigeon and chicken ciliary ganglia at a concentration of 10 .mu.g/ml (1.2 .mu.M). The blockade is antagonized by pre-incubation with 100 .mu.M tubocurarine. Further evidence that this blockade is produced by a postsynaptic action, as one would expect of an .alpha.-neurotoxin, are findings that: exposure to the toxin prevents the depolarization of ganglion cells normally seen in response to the cholinergic agonist, carbachol; and the blocking activity of the toxin is removed by treatment with membranes purified from Torpedo electric organ containing an excess of .alpha.-neurotoxin binding sites. A high affinity binding site for [125I].alpha.-bungarotoxin was characterized in the chicken ciliary ganglion. Since it is labeled equally well by lots of .alpha.-bungarotoxin which block transmission and those that do not, this site does not appear to be involved in the blockade of transmission. .alpha.-Cobratoxin (from Naja naja siamensis), the .alpha.-neurotoxin L. s. III (from Laticauda semifasciata) and certain lots of .alpha.-bungarotoxin produce a partial bllockade of transmission in ciliary neurons of the pigeon ciliary ganglion at a concentration of 10 .mu.g/ml (1.2 .mu.M), but have no effect on transmission in choroid neurons. Two other .alpha.-neurotoxins from L. semifasciata, erabutoxin a and erabutoxin b, have no effect on transmission in either cell population at this concentration. None of the .alpha.-neurotoxins tested had any effect on transmission in either the rat superior cervical ganglion or the rat pelvic ganglion at concentrations up to 100 .mu.g/ml (12 .mu.M). Collagenase treatment of these ganglia to increase access of the toxins to ganglion cells did not alter these negative results. .beta.-Bungarotoxin (0.5 .mu.g/ml, 0.02 .mu.M) produces a complex blockade of transmission in both avian ciliary ganglia and rat superior cervical ganglia. Unlike the action of .alpha.-bungarotoxin, the blockade of ciliary ganglion transmission by .beta.-bungarotoxin is irreversible and is not prevented by pretreatment with tubocurarine.