Mycobacterium tuberculosis H37Rv induces ectosome release in human polymorphonuclear neutrophils
Mycobacterium tuberculosis H37Rv induces ectosome release in human polymorphonuclear neutrophils
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DOI：
10.1016/j.tube.2010.01.002
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发表时间：
2010-03-01
期刊：
影响因子：
3.2
通讯作者：
Estrada-Garcia, Iris
中科院分区：
文献类型：
--
作者：
Gonzalez-Cano, Patricia;Mondragon-Flores, Ricardo;Estrada-Garcia, Iris
细胞外吞作用是真核细胞进行分子信息交换的一种重要现象，是细胞外体释放的过程。从N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（fMLP）激活的人多形核中性粒细胞（PMNs）释放的Ects最近已被表征。在这些囊泡中发现了分子如CD 35和磷脂酰丝氨酸（PS），以及酶如髓过氧化物酶和弹性蛋白酶，表明来自PMN的Ects可以作为具有抗微生物活性的外细胞器。在这里，我们第一次表明，人类中性粒细胞释放外泌体在结核分枝杆菌H37 Rv感染。我们发现，外体的释放并不完全与分枝杆菌感染有关，因为感染了其他微生物（如：例如，在一个实施例中，墨西哥利什曼原虫、金黄色葡萄球菌和大肠杆菌或用佛波醇肉豆蔻酸酯乙酸酯（PMA）活化）也诱导胞吞作用。感染或激活后10 min开始释放ECTS。在Ects膜上显示了CD 35、PS、Rab 5、Rab 7和Cyt b555亚基gp 91（Phox）的表达。根据我们的观察，我们得出结论，Ects释放从人类中性粒细胞在细胞活化的反应，这一过程是不相关的凋亡。(C)2010爱思唯尔有限公司版权所有。
Ectocytosis, the cellular process by which ectosomes (Ects) are released, is an important phenomenon by which eukaryotic cells exchange molecular information. Ects released from N-formylmethionyl-leucyl-phenylalanine (fMLP)-activated human polymorphonuclear neutrophils (PMNs) have recently been characterized. Molecules such as CD35 and phosphatidylserine (PS), and enzymes such as myeloperoxidase and elastase were found in these vesicles, suggesting that Ects from PMNs could function as ecto-organelles with anti-microbial activity. Here we show for the first time that human PMNs release ectosomes in response to Mycobacterium tuberculosis H37Rv infection. We found that the release of ectosomes was not associated exclusively with mycobacterial infection since infection with other microorganisms (e. g., Leishmania mexicana, Staphylococcus aureus, and Escherichia coli or activation with phorbol myristate acetate (PMA)) also induced ectocytosis. Ects release started as early as 10 min after infection or activation. Expression of CD35, PS, Rab5, Rab7 and gp91(Phox), a subunit of Cyt b555 was demonstrated on the Ects membrane. Based on our observations we conclude that Ects are released from human neutrophils in response to cell activation and that this process is not related to apoptosis. (C) 2010 Elsevier Ltd. All rights reserved.