Nitrogen and methanogen community composition within and among three Sphagnum dominated peatlands in Scandinavia
Nitrogen and methanogen community composition within and among three Sphagnum dominated peatlands in Scandinavia
复制标题
DOI：
10.1016/j.soilbio.2014.11.016
复制
发表时间：
2015-02
影响因子：
9.7
通讯作者：
M. Martí;H. Juottonen;B. Robroek;K. Yrjälä;Å. Danielsson;P. Lindgren;B. Svensson
中科院分区：
文献类型：
--
作者：
M. Martí;H. Juottonen;B. Robroek;K. Yrjälä;Å. Danielsson;P. Lindgren;B. Svensson
富营养化的上升沼泽是营养贫乏的酸性泥炭地，积累有机物质。它们广泛分布在北方纬度，是向大气排放甲烷的重要来源，从气候变化的角度来看，这是一个非常令人关切的问题。我们调查了产甲烷菌群落组成沿着微地形梯度内三个沼泽在斯堪的纳维亚半岛，接收不同数量的氮沉淀。themcrA基因的末端限制性片段长度多态性的产甲烷群落分析表明，三个网站之间的不同配置文件，而没有观察到的微地形梯度的影响。泥炭温度和溶解有机碳是主要的土壤变量解释38%的沼泽中的甲烷群落多样性的变化。家庭Methanoregulaceae（氢营养产甲烷菌）表现出最大的相对比例和最高的活动在所有三个网站。themcrA基因和转录本的定量PCR显示，最北方站点，接收最低的大气氮负荷，具有显着较低的丰度和活性的甲烷菌（4.7 × 106和2.4 × 104 mcrAcopies/g土壤），与最南部站点相比氮素负荷最高的是两种土壤，分别为8.2 × 107和4.6 × 105 mcr/g土。没有模式的themcrA基因和转录丰度观察沿着微地形。结果表明，产甲烷菌的出现差异主要是由于地质气候条件，而不是现场固有的微地形变化。该研究进一步表明，该网站的内在地形的环境变化不会影响产甲烷活性，而在斯堪的纳维亚的富营养化提出沼泽平均温度的增加可能有助于增加产甲烷古菌的活动，导致甲烷产量的增加。
Ombrotrophic raised bogs are nutrient poor acidic peatlands accumulating organic matter. They are widely spread on northern latitudes and are substantial sources of methane emissions to the atmosphere being of great concern from a climate change perspective. We investigated the methanogen community composition along microtopographic gradients within three bogs in Scandinavia, receiving different amounts of nitrogen precipitation. Methanogenic community analyses by terminal restriction fragment length polymorphism of themcrAgene showed different profiles among the three sites, while no influence of the microtopographic gradients was observed. Peat temperature and dissolved organic carbon were the major edaphic variables explaining 38% of the variation of the methanogenic community diversity among the bogs. The familyMethanoregulaceae(hydrogenotrophic methanogens) showed the largest relative proportion and highest activity in all three sites. Quantitative PCR of themcrAgene and transcripts showed that the most northern site, receiving the lowest atmospheric nitrogen load, had significantly lower abundance and activity of methanogens (4.7 × 106and 2.4 × 104mcrAcopies per gram of soil, respectively), compared to the most southern site (8.2 × 107and 4.6 × 105mcrAcopies per gram of soil, respectively), receiving the highest nitrogen load. No patterns of themcrAgene and transcript abundances were observed along the microtopography. The results indicated that the difference in occurrence of methanogens is mainly due to geoclimatological conditions rather than site intrinsic microtopographic variation. The study further suggests that environmental changes on the site intrinsic topography will not affect the methanogenic activity, while increasing average temperatures in Scandinavian ombrotrophic raised bogs might contribute to an increase of the methanogenic archaeal activity resulting in an increase of methane production.