Allograft inflammatory factor-1 alleviates liver disease of BALB/c mice infected with Schistosoma japonicum
Allograft inflammatory factor-1 alleviates liver disease of BALB/c mice infected with Schistosoma japonicum
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同种异体移植炎症因子1减轻日本血吸虫感染的BALB/c小鼠的肝病
DOI：
10.1007/s00436-014-3915-8
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发表时间：
2014-07-01
影响因子：
2
通讯作者：
Niu, An-Ou
中科院分区：
文献类型：
--
作者：
Chen, Qiong-Rong;Guan, Fei;Niu, An-Ou
同种异体移植物炎症因子-1(AIF-1)在多种炎症状态下发挥重要作用。我们先前的研究表明，AIF-1在感染日本血吸虫的BALB/c小鼠的肝脏中过表达，并在血吸虫病的发病机制中发挥重要作用。本研究旨在探讨AIF-1治疗对日本血吸虫感染BALB/c小鼠肝纤维化和肝坏死的影响。72只BALB/c小鼠感染日本血吸虫尾虫，随机分为3组：AIF-1组、生理盐水组和对照组。分别于感染后5、8、14周观察小鼠的生命体征、肝功能、虫卵负荷和肝脏病理变化，并检测肝、脾组织中肿瘤坏死因子-1和肿瘤坏死因子-α的水平。日本血吸虫感染小鼠经AIF-1治疗14周后，肝、脾组织中肿瘤坏死因子-α的表达均受到抑制，而肝、脾组织中AIF-1的作用增强。组织病理学分析和肝组织Masson三色染色结果显示，与其他感染组相比，感染后14周小鼠肝组织纤维化和坏死明显减轻。AIF-1对感染日本血吸虫小鼠的治疗可减轻肝纤维化和坏死，提示AIF-1可防治肝纤维化。
Allograft inflammatory factor-1 (AIF-1) plays an important role in various inflammatory conditions. Our previous study demonstrated that AIF-1 was over-expressed in the liver of BALB/c mice infected with Schistosoma japonicum and played significant role in the pathogenesis of schistosomiasis. The aim of this study was to focus on the effect of AIF-1 treatment on liver fibrosis and necrosis of BALB/c mice infected with S. japonicum. Seventy-two BALB/c mice were infected with cercariae of S. japonicum and then divided into three groups: AIF-1-treated group, saline-treated group, and control group. The vital signs, liver function, egg load, and hepatic pathological changes of the mice were assessed, and the levels of AIF-1 and TNF-α in the liver and spleen were measured at 5, 8, and 14 weeks postinfection. The treatment of AIF-1 on the mice infected with S. japonicum suppressed the expression of TNF-α and increased the effectiveness of AIF-1 in the liver and spleen at 14 weeks postinfection. Histopathological analysis and Masson trichrome staining for the liver tissues showed that the liver fibrosis and necrosis were alleviated previously compared with other infected mice at 14 weeks postinfection. The treatment of AIF-1 on the mice infected with S. japonicum can alleviate hepatic fibrosis and necrosis which indicate that AIF-1 use may prevent and cure the liver fibrosis.