Phylogenetic analysis of bovine viral diarrhea viruses using five different genetic regions
Phylogenetic analysis of bovine viral diarrhea viruses using five different genetic regions
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DOI：
10.1016/j.virusres.2003.10.006
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发表时间：
2004-02-01
期刊：
影响因子：
5
通讯作者：
Akashi, H
中科院分区：
文献类型：
--
作者：
Nagai, M;Hayashi, M;Akashi, H
对48株日本和已报道的牛病毒性腹泻病毒（BVDV）基因组的5个不同区域（5'非编码区（5' NCR）、N-pro、E2、NS 3和NS 5 B-3 'NCR）进行了系统发育分析。将日本BVDV分为BVDV 1（细分为6个亚组）和BVDV 2。1975年分离到的一个分离物So CP/75，根据其5 'NCR序列被认为是1 e亚群，它是一个非常独特的分离物，在任何地区的树中都形成了一个独立的谱系。从实验性粘膜病病例中获得的另一个分离物190 CP被分类为亚组1 e，由Becher等人在5 'NCR、N-pro和E2区域中定义，而它在NS 5 B-3' NCR区域中被分类为亚组la。从GenBank数据库中获得的美国分离株ILLC和ILLNC的基因组序列在5 'NCR、N-pro、E2和NS 5 B-3' NCR区域中被分配到亚组1b，而它们在NS 3区域中被分配到亚组1a，这表明在动物中发生了被分类为不同亚组的病毒株之间的重组。这些结果表明，几个遗传区域的系统发育分析可用于进一步表征田间BVDV分离株。(C)2003 Elsevier B. V.保留所有权利。
Phylogenetic analysis of the five different regions (5' non-coding region (5'NCR), N-pro, E2, NS3 and NS5B-3'NCR) of 48 Japanese and reported bovine viral diarrhea virus (BVDV) genomes was performed. Japanese BVDVs were segregated into BVDV1 subdivided into six subgroups and BVDV2. One isolate, So CP/75, isolated in 1975 and previously proposed as subgroup 1e according to its 5'NCR sequence, was quite unique and formed an independent lineage in the tree of any region. Another isolate, 190CP, obtained from an experimental mucosal disease case was classified as subgroup 1e, defined by Becher et al. in the 5'NCR, N-pro and E2 regions, whereas it was classified as subgroup la in the NS5B-3'NCR region. The genomic sequences of the American isolates ILLC and ILLNC obtained from the GenBank database were assigned into subgroup 1b in the 5'NCR, N-pro, E2 and NS5B-3'NCR regions, whereas they were assigned into subgroup la in the NS3 region, suggesting that recombination between the virus strains classified into different subgroups had occurred in an animal. These findings suggest that phylogenetic analysis of several genetic regions is useful for the further characterization of field BVDV isolates. (C) 2003 Elsevier B.V. All rights reserved.