Activation of the Arabidopsis thaliana Mitogen-Activated Protein Kinase MPK11 by the Flagellin-Derived Elicitor Peptide, flg22
Activation of the Arabidopsis thaliana Mitogen-Activated Protein Kinase MPK11 by the Flagellin-Derived Elicitor Peptide, flg22
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DOI：
10.1094/mpmi-11-11-0281
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发表时间：
2012-04-01
影响因子：
3.5
通讯作者：
Lee, Justin
中科院分区：
文献类型：
--
作者：
Bethke, Gerit;Pecher, Pascal;Lee, Justin
丝裂原活化蛋白激酶（MAPK）介导的细胞信号转导过程中的压力反应，以及在真核生物的各种生长和发育过程。已知病原体感染或用保守的病原体相关分子模式（PAMP）如细菌鞭毛蛋白衍生的flg22肽处理可激活三种拟南芥MAPK：MPK 3、MPK 4和MPK 6。已知包括flg22处理在内的几种应激增加MPK11表达，但尚未显示MPK11的活化。在这里，我们表明，MPK11活性确实可以通过flg22诱导增加。一个小规模的微阵列分析防御相关基因显示，肉桂醇脱氢酶5需要MPK11的完整flg22诱导的表达。一个mpk11突变体表现出增加flg22介导的生长抑制，但没有改变的敏感性假单胞菌，灰葡萄孢，或Alternaria brassicicicola。在mpk3、mpk6或mpk4背景中，MPK11是胚胎或种子发育或一般生存力所必需的。虽然这种发育缺陷的双突变体和缺乏或只有微妙的mpk11表型表明功能MAPK冗余，与旁系同源MPK4的比较揭示了不同的功能。总之，MAPK在应激信号转导中的作用的未来调查应包括MPK11作为第四个PAMP激活的MAPK。
Mitogen-activated protein kinases (MAPK) mediate cellular signal transduction during stress responses, as well as diverse growth and developmental processes in eukaryotes. Pathogen infection or treatments with conserved pathogen-associated molecular patterns (PAMPs) such as the bacterial flagellin-derived flg22 peptide are known to activate three Arabidopsis thaliana MAPK: MPK3, MPK4, and MPK6. Several stresses, including flg22 treatment, are known to increase MPK11 expression but activation of MPK11 has not been shown. Here, we show that MPK11 activity can, indeed, be increased through flg22 elicitation. A small-scale microarray for profiling defense-related genes revealed that cinnamyl alcohol dehyrogenase 5 requires MPK11 for full flg22-induced expression. An mpk11 mutant showed increased flg22-mediated growth inhibition but no altered susceptibility to Pseudomonas syringae, Botrytis cinerea, or Alternaria brassicicola. In mpk3, mpk6, or mpk4 backgrounds, MPK11 is required for embryo or seed development or general viability. Although this developmental deficiency in double mutants and the lack of or only subtle mpk11 phenotypes suggest functional MAPK redundancies, comparison with the paralogous MPK4 reveals distinct functions. Taken together, future investigations of MAPK roles in stress signaling should include MPK11 as a fourth PAMP-activated MAPK.