IRON AND ALUMINUM IN RELATION TO BRAIN FERRITIN IN NORMAL INDIVIDUALS AND ALZHEIMERS-DISEASE AND CHRONIC RENAL-DIALYSIS PATIENTS
IRON AND ALUMINUM IN RELATION TO BRAIN FERRITIN IN NORMAL INDIVIDUALS AND ALZHEIMERS-DISEASE AND CHRONIC RENAL-DIALYSIS PATIENTS
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DOI：
10.1042/bj2870509
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发表时间：
1992-10-15
影响因子：
4.1
通讯作者：
HARRISON, PM
中科院分区：
文献类型：
--
作者：
DEDMAN, DJ;TREFFRY, A;HARRISON, PM
铁蛋白已被分离，其亚单位组成，铁和铝含量测定在大脑皮层和小脑的正常人和大脑皮层的阿尔茨海默氏病和肾透析患者。一个地球解放军。并测定了正常人和阿尔茨海默病患者顶叶皮质的铁蛋白、非血红素铁和铝含量。结果发现，正常人大脑皮层和小脑的铁蛋白具有高的H-亚基含量，与心脏铁蛋白相似。从大脑皮层分离的铁蛋白的亚基组成在阿尔茨海默病或肾透析患者中没有显着改变。正常人大脑皮层中的铁蛋白只有大约。1500个铁原子/分子和铁蛋白的铁含量在阿尔茨海默病或肾透析患者中没有显着变化。从正常人、阿尔茨海默病患者和肾透析患者的大脑皮层中分离出的铁蛋白每个分子中的铝原子少于9个。在大脑皮层中铝浓度显著增加的透析患者中，未能发现与铁蛋白相关的铝浓度增加，表明铝不会在体内铁蛋白中蓄积。这对铝的毒性具有重要意义，因为它意味着细胞不能通过与铁相同的机制来解毒铝。比较铁蛋白，铝和铁在正常和阿尔茨海默病患者的顶叶皮层的浓度表明，而铝的浓度没有增加，铁蛋白和铁显着增加阿尔茨海默病。
Ferritin has been isolated and its subunit composition, iron and aluminium content determined in the cerebral cortex and cerebellum of normal individuals and in the cerebral cortex of Alzheimer's-disease and renal-dialysis patients. An e.l.i.s.a. for ferritin has been developed and the ferritin, non-haem iron and aluminium content of the parietal cortex were determined in normal individuals and Alzheimer's-disease patients. It was found that ferritin from the cerebral cortex and cerebellum of normal individuals had a high H-subunit content, similar to that of heart ferritin. The subunit composition of ferritin isolated from the cerebral cortex was not significantly altered in Alzheimer's-disease or renal-dialysis patients. Ferritin from the cerebral cortex of normal individuals had only approx. 1500 atoms of iron per molecule and the iron content of ferritin was not significantly changed in Alzheimer's-disease or renal-dialysis patients. Ferritin isolated from the cerebral cortex of normal, Alzheimer's-disease and renal-dialysis patients had less than 9 atoms of aluminium per molecule. The failure to find increased concentrations of aluminium associated with ferritin in dialysis patients, who had markedly increased concentrations of aluminium in the cerebral cortex, shows that aluminium does not accumulate in ferritin in vivo. This has important implications for the toxicity of aluminium, since it implies that cells are unable to detoxify aluminium by the same mechanism as that available for iron. Comparison of the concentrations of ferritin, aluminium and iron in the parietal cortex from normal and Alzheimer's-disease patients showed that, whereas the concentration of aluminium was not increased, both ferritin and iron were significantly increased in Alzheimer's disease.