New genomospecies related to Borrelia valaisiana, isolated from mammals in Okinawa archipelago, Japan
New genomospecies related to Borrelia valaisiana, isolated from mammals in Okinawa archipelago, Japan
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DOI：
10.1099/jmm.0.05337-0
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发表时间：
2004-05-01
影响因子：
3
通讯作者：
Imai, Y
中科院分区：
文献类型：
--
作者：
Masuzawa, T;Hashimoto, N;Imai, Y
在2000年和2001年，对从日本最南端的岛屿，包括冲绳（主岛）、Izena、西表岛和石垣岛的各种哺乳动物中分离出的疏螺旋体进行了调查。采用5S-23 SrDNA基因间隔区的RFLP分析和基因间隔区、16 SrDNA和鞭毛蛋白基因的序列分析方法，对110个臭鼩（Suncus murinus）、4个小家鼠（Mus calori）、1个褐家鼠（Rattus norvegicus）和1个大鳄鱼（Crocidura watasei）的耳组织分离物进行了鉴定。虽然这些分离株与在韩国、台湾和中国南部和中部地区发现的瓦氏疏螺旋体显示相同的RFLP，但它们的RFLP模式与B的不同。valaisiana和Am 501分离自日本的Nodes columnostrium。根据鞭毛蛋白基因和16 S rDNA序列构建的系统发育树表明，这些菌株聚在一起，但与欧洲菌株B的亲缘关系较远。valaisiana和菌株Am 501。这些结果表明，这些分离株在东亚发现应被归类为一个新的基因型伯氏疏螺旋体广义。
In 2000 and 2001, a survey was conducted of Borrelia isolated from various mammals in the southernmost islands of Japan, including Okinawa (main island), Izena, Iriomote and Ishigaki. Isolates obtained from the ear tissues of Suncus murinus (110 isolates), Mus calori (four isolates), Rattus norvegicus (one isolate) and Crocidura watasei (one isolate), were characterized by RFLP of the 5S-23S rDNA intergenic spacer and sequence analysis of the intergenic spacer, 16S rDNA and flagellin gene. While these isolates showed identical RFLPs to Borrelia valaisiana found in Korea, Taiwan and the southern and central parts of China, their RFLP patterns differed from those of B. valaisiana found in European countries, and strain Am501 isolated from Nodes columnae in Japan. It was found that these isolates clustered with each other on a phylogenetic tree based on flagellin gene and 16S rDNA sequences, but were relatively divergent from the European B. valaisiana and strain Am501. These findings suggest that these isolates found in East Asia should be classified as a new genomospecies of Borrelia burgdorferi sensu lato.