Maturational changes in diffusion anisotropy in the rat corpus callosum: Comparison with quantitative histological evaluation
Maturational changes in diffusion anisotropy in the rat corpus callosum: Comparison with quantitative histological evaluation
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DOI：
10.1002/jmri.21496
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发表时间：
2008-10-01
影响因子：
4.4
通讯作者：
Inubushi, Toshiro
中科院分区：
文献类型：
--
作者：
Jito, Junya;Nakasu, Satoshi;Inubushi, Toshiro
目的：确定影响生后发育过程中各向异性分数(FA)的主要组织成分，并将FA值与定量评价的组织学变化进行比较。材料和方法：用7.0T磁共振成像仪获取出生后1~10周的大鼠胼胝体的弥散张量成像(DTI)数据。甲苯胺蓝染色半薄切片定量评价组织学参数。结果：髓鞘面积与平均FA值呈高度正相关(r=0.856，P<0.01)，出生后4周后，FA值变化较小。细胞外间隙面积与微血管密度呈负相关(r=-0.813.0 5，P<0.0 1)。在前向逐步分析中，髓鞘面积对MFA的影响最大(P<；0.001)，其次是无髓轴突数量(P=0.113)。多元线性回归分析显示，这两个参数对MFA的预测具有极显著的调整相关系数(r(2)adj.=0.738，P<；0.001)。结论：在大鼠穹隆发育早期，髓鞘面积对FA值的贡献最大。
Purpose: To determine the main histological components that affect fractional anisotropy (FA) in postnatal development of the rat corpus callosum and compare FA values with histological changes evaluated quantitatively.Materials and Methods: Diffusion tensor image (DTI) data of the rat (postnatal 1-10 weeks) corpus callosum were obtained with a 7.0 T MR scanner. Histological parameters were quantitatively assessed in toluidine blue-stained semithin sections. Simple and multiple linear regression analyses were performed to investigate relationships between FA values and histological variables.Results: The mean FA value (mFA) increased significantly in the early growth stages, whereas the change became smaller after postnatal week 4. Simple regression analysis showed a high correlation between the area of myelin sheath and mFA (r = 0.856; P < 0.01). The area of extracellular space correlated negatively with mFA (r = -0.813; P < 0.01). In a forward stepwise analysis, the area of myelin sheath had the strongest influence on mFA (P < 0.001), followed by the number of unmyelinated axons (P = 0.113). Multiple linear regression analysis revealed that both parameters predicted mFA with a highly significant adjusted correlation coefficient (r(2) adj. = 0.738, P < 0.001).Conclusion: During the early development stage in the rat corpus callosum, the strongest contribution to FA value is the area of myelin sheath.