THE PHYLOGENY OF HUMAN-CHROMOSOME SPECIFIC ALPHA-SATELLITES
THE PHYLOGENY OF HUMAN-CHROMOSOME SPECIFIC ALPHA-SATELLITES
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DOI：
10.1007/bf00303039
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发表时间：
1988-01-01
期刊：
影响因子：
1.6
通讯作者：
YUROV, YB
中科院分区：
文献类型：
--
作者：
ALEXANDROV, IA;MITKEVICH, SP;YUROV, YB
通过原位杂交，确定了18个锥形α卫星片段的同源序列的染色体分布。所有克隆的序列似乎都是位于单染色体对上的小重复家族成员。在研究的序列中，发现了位于第3、4、10、11、17、18和X染色体上的特异分子标记。通过比较在非严格条件下获得的杂交光谱和不同染色体特异性家系中的限制酶切点周期，可以鉴定出三个“超染色体”家系，每个家系位于一组特有的染色体上。这三个家族共同覆盖了所有常染色体和X染色体。这些数据加上之前报道的数据，可以绘制出染色体特有的阿尔法卫星重复序列的部分系统发育树。每个超染色体家族都可能起源于不同的祖先序列，并由某些类型的单体组成。通过染色体间转移和随后的扩增事件的循环，祖先序列已经进化成许多染色体特有的家族。染色体特异家族的高度同质性可能是染色体特异的α卫星结构域中扩增单位的染色体内同质性的结果。
The chromosomal distribution of sequences homologous to 18 coned alpha satellite fragments was established by in situ hybridization. It appeared that all the cloned sequences were members of small repeated families located on single chromosome pairs. Among the sequences studied specific molecular markers for chromosomes 3, 4, 10, 11, 17, 18 and X were found. Comparison of the hybridization spectra obtained under non-stringent conditions and of restriction site periodicities in different chromosome-specific families allowed the identification of three "suprachromosomal" families, each located on a characteristic set of chromosomes. The three families together cover all the autosomes and the X chromosome. These data plus those reported previously allow part of the phylogenetic tree of chromosome-specific alpha satellite repeats to be drawn. Each suprachromosomal family has presumably originated from a distinct ancestral sequence and consists of certain types on monomers. Ancestral sequences have evolved into a number of chromosome-specific families by cycles of interchromosomal transfers and subsequent amplification events. The high homogeneity of chromosome-specific families may be a result of intrachromosomal homogenization of amplification units in chromosome-specific alpha satellite domains.