β-Adrenergic regulation of IL-6 release from adipose tissue: In vivo and in vitro studies
β-Adrenergic regulation of IL-6 release from adipose tissue: In vivo and in vitro studies
复制标题
DOI：
10.1210/jcem.86.12.8104
复制
发表时间：
2001-12
期刊：
影响因子：
--
通讯作者：
V. Mohamed-ali;L. Flower;Jaswinder K. Sethi;G. Hotamisligil;R. Gray;S. Humphries;D. York;J. Pinkney
中科院分区：
文献类型：
--
作者：
V. Mohamed-ali;L. Flower;Jaswinder K. Sethi;G. Hotamisligil;R. Gray;S. Humphries;D. York;J. Pinkney
肥胖患者循环IL-6水平升高。虽然IL-6在脂肪组织中表达，但其调控和细胞起源都没有很好的表征。本研究结合人、动物体内和体外培养脂肪细胞研究，探讨β-肾上腺素能对IL-6释放的调节作用。人体体内研究：人体志愿者输注异丙肾上腺素、去甲肾上腺素或生理盐水{4 m:4F；平均（sd）年龄35.5（5.8）岁；身体质量指数24.6 (4.2)kg/m−2}。血浆IL-6水平在异丙肾上腺素输注3 h时升高（P = 0.01），输注2 h后下降（P = 0.05）。IL-6水平在去甲肾上腺素和生理盐水组均无显著变化。小鼠体内实验：C57BL6/J雄性小鼠分别注射多巴酚丁胺（β1激动剂）、克仑特罗（β2）、CL316243 （β3）或生理盐水安慰剂。与安慰剂相比，克仑特罗组3 h血浆IL-6水平升高（P = 0.02）， CL316243组升高（P = 0.02），但多巴酚丁胺组没有升高（P = 0.51）。体外研究：在人外周血细胞中…
Circulating IL-6 levels are elevated in obesity. Although IL-6 is expressed in adipose tissue, neither its regulation nor cell of origin is well characterized. Here we investigated the β-adrenergic regulation of IL-6 release in a combination of studies on humans and animals in vivo and cultured adipocytes in vitro. Human in vivo study: Human volunteers were infused with isoproterenol, norepinephrine, or saline {4 m:4F; mean (sd) age 35.5 (5.8) yr; body mass index 24.6 (4.2) kg/m−2}. Plasma IL-6 levels increased during a 3-h infusion of isoproterenol (P = 0.01) and fell 2 h post infusion (P = 0.05). IL-6 levels did not change significantly with either norepinephrine or saline. Murine in vivo study: C57BL6/J male mice were injected ip with dobutamine (β1 agonist), clenbuterol (β2), CL316243 (β3), or saline placebo. Plasma IL-6 levels at 3 h were increased by clenbuterol (P = 0.02) and CL316243 (P = 0.02) but not dobutamine (P = 0.51), compared with placebo. In vitro studies: In human peripheral blood cells,...