Evaluation of bacteria isolated from rice for plant growth promotion and biological control of seedling disease of rice.
Evaluation of bacteria isolated from rice for plant growth promotion and biological control of seedling disease of rice.
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DOI：
10.1139/w01-097
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发表时间：
2001-10
影响因子：
2.8
通讯作者：
T. Adhikari;C. M. Joseph;Guo-Ping Yang;Donald A. Phillips;Louise M. Nelson
中科院分区：
文献类型：
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作者：
T. Adhikari;C. M. Joseph;Guo-Ping Yang;Donald A. Phillips;Louise M. Nelson
从加州分离的102株水稻根际细菌和内生细菌中，有37%的细菌在离体条件下对引起水稻苗期病害的克氏Achlya klebsiana和刺腐霉Pythium spinosum的生长有显著抑制作用（P <或= 0.05）。4株内生菌在生长袋试验中对幼苗病害非常有效，通过扩增的16 S rRNA基因测序，这些菌株被鉴定为荧光假单胞菌（S3）、托氏假单胞菌（S20）、韦氏假单胞菌（S21）和真鞘氨醇单胞菌（S12）。菌株S12、S20和S21含有固氮基因nifD，但只有S12能够在纯培养中还原乙炔。在没有病原体的情况下，这四个菌株显着增强了植物的生长，接种水稻幼苗的株高和干重相对于未接种水稻的增加就证明了这一点。在盆栽生物测定中评价了三个细菌菌株（S3、S20和S21），并且降低了50%-73%的发病率。菌株S3在最低接种密度（106 CFU/mL）和较高密度（10（8）CFU/mL或未稀释悬浮液）下抑制疾病的效果相同。本研究表明，所筛选的内生细菌菌株具有防治水稻苗期病害和促进植物生长的潜力。
Of 102 rhizoplane and endophytic bacteria isolated from rice roots and stems in California, 37% significantly (P < or = 0.05) inhibited the growth in vitro of two pathogens, Achlya klebsiana and Pythium spinosum, causing seedling disease of rice. Four endophytic strains were highly effective against seedling disease in growth pouch assays, and these were identified as Pseudomonas fluorescens (S3), Pseudomonas tolaasii (S20), Pseudomonas veronii (S21), and Sphingomonas trueperi (S12) by sequencing of amplified 16S rRNA genes. Strains S12, S20, and S21 contained the nitrogen fixation gene, nifD, but only S12 was able to reduce acetylene in pure culture. The four strains significantly enhanced plant growth in the absence of pathogens, as evidenced by increases in plant height and dry weight of inoculated rice seedlings relative to noninoculated rice. Three bacterial strains (S3, S20, and S21) were evaluated in pot bioassays and reduced disease incidence by 50%-73%. Strain S3 was as effective at suppressing disease at the lowest inoculum density (106 CFU/mL) as at higher density (10(8) CFU/mL or undiluted suspension). This study indicates that selected endophytic bacterial strains have potential for control of seedling disease of rice and for plant growth promotion.