Technical challenges in the isolation and analysis of circulating tumor cells.
Technical challenges in the isolation and analysis of circulating tumor cells.
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DOI：
10.18632/oncotarget.11191
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发表时间：
2016-09-20
期刊：
影响因子：
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通讯作者：
Pienta KJ
中科院分区：
文献类型：
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作者：
van der Toom EE;Verdone JE;Gorin MA;Pienta KJ
越来越多的证据表明，癌细胞在全身治疗后表现出动态的分子变化。外周血中的循环肿瘤细胞(CTC)代表了一种现成的癌细胞来源，可以用来测量这一动态过程。到目前为止，已经描述了大量分离和表征四氯化碳的策略。然而，这些技术在灵敏和特异地检测这些稀有细胞的能力方面都有独特的局限性。在这篇综述中，我们重点讨论了最常见的四氯化碳分离和检测策略的技术限制和缺陷。此外，我们强调了使用常见的生物标志物正确地将稀有细胞分类为CTCs的困难。至于未来制定的化验方法，第一步必须是统一和明确地界定根据疾病特定生物标记物将一个物体指定为四氯化碳的标准。
Increasing evidence suggests that cancer cells display dynamic molecular changes in response to systemic therapy. Circulating tumor cells (CTCs) in the peripheral blood represent a readily available source of cancer cells with which to measure this dynamic process. To date, a large number of strategies to isolate and characterize CTCs have been described. These techniques, however, each have unique limitations in their ability to sensitively and specifically detect these rare cells. In this review we focus on the technical limitations and pitfalls of the most common CTC isolation and detection strategies. Additionally, we emphasize the difficulties in correctly classifying rare cells as CTCs using common biomarkers. As for assays developed in the future, the first step must be a uniform and clear definition of the criteria for assigning an object as a CTC based on disease-specific biomarkers.