Curved FtsZ protofilaments generate bending forces on liposome membranes
Curved FtsZ protofilaments generate bending forces on liposome membranes
复制标题
DOI：
10.1038/emboj.2009.277
复制
发表时间：
2009-11-18
期刊：
影响因子：
11.4
通讯作者：
Erickson, Harold P.
中科院分区：
文献类型：
--
作者：
Osawa, Masaki;Anderson, David E.;Erickson, Harold P.
我们已经创建了FtsZ-YFP-mts，其中C-末端上的两亲性螺旋将FtsZ拴系到膜上。当掺入多层管状脂质体内时，FtsZ-YFP-mts可以组装产生收缩力的Z环。当添加到脂质体外部时，FtsZ-YFP-mts结合并产生凹陷，使膜以与脂质体内部的Z环相同的方向弯曲。突出的膜小管，然后挤出的交叉处的凹陷。我们测试了将膜靶向序列（mts）从C-末端移动到N-末端的效果，N-末端与C-末端系链成大约180度。当将mts-FtsZ-YFP施加到脂质体的外部时，其产生凸起，使膜在与凹陷凹陷相反的方向上弯曲。我们的结论是，FtsZ原丝有一个固定的方向的曲率，和膜弯曲的方向取决于哪一侧的弯曲原丝的MTS是连接。这支持FtsZ收缩力由原丝弯曲产生的模型。The EMBO Journal（2009）28，3476-3484. doi：10.1038/doj.2009.277; 2009年9月24日在线发布
We have created FtsZ-YFP-mts where an amphipathic helix on the C-terminus tethers FtsZ to the membrane. When incorporated inside multi-lamellar tubular liposomes, FtsZ-YFP-mts can assemble Z rings that generate a constriction force. When added to the outside of liposomes, FtsZ-YFP-mts bound and produced concave depressions, bending the membrane in the same direction as the Z ring inside liposomes. Prominent membrane tubules were then extruded at the intersections of concave depressions. We tested the effect of moving the membrane-targeting sequence (mts) from the C-terminus to the N-terminus, which is approximately 180 degrees from the C-terminal tether. When mts-FtsZ-YFP was applied to the outside of liposomes, it generated convex bulges, bending the membrane in the direction opposite to the concave depressions. We conclude that FtsZ proto-filaments have a fixed direction of curvature, and the direction of membrane bending depends on which side of the bent protofilament the mts is attached to. This supports models in which the FtsZ constriction force is generated by protofilament bending. The EMBO Journal (2009) 28, 3476-3484. doi:10.1038/emboj.2009.277; Published online 24 September 2009