A Flexible Aptameric Graphene Field-Effect Nanosensor Capable of Automatic Liquid Collection/Filtering for Cytokine Storm Biomarker Monitoring in Undiluted Sweat
A Flexible Aptameric Graphene Field-Effect Nanosensor Capable of Automatic Liquid Collection/Filtering for Cytokine Storm Biomarker Monitoring in Undiluted Sweat
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DOI：
10.1002/adfm.202309447
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发表时间：
2023-11-27
影响因子：
19
通讯作者：
Pan，Yunlu
中科院分区：
文献类型：
--
作者：
Huang，Cong;Li，Dongliang;Pan，Yunlu
一种能够直接检测人体外部分泌物中的细胞因子的可穿戴传感器对于测量免疫反应的强度和规避炎症风暴至关重要，这是一个重要的临床问题。由于分泌物中杂质的干扰，低浓度细胞因子的检测常常受到阻碍。为了解决这一挑战，研究者开发了一种可穿戴检测系统，该系统集成了具有水自动收集/过滤功能的Janus膜和适配体修饰的石墨烯场效应晶体管传感器，能够检测未稀释汗液样本中的低浓度细胞因子。Janus膜可以自动过滤外部分泌物并将其输送到传感器表面，有效消除杂质干扰，增强传感器的检测能力。该设备可以灵敏地检测未稀释汗液中的细胞因子（如TNF‐α，炎症和癌症的标志物），检测范围为0.5至500 pM，检测限为0.31 pM。该传感器的超灵活性也得到了验证，即使在承受高达50次极端变形后也能保持稳定的电气性能。这些发现表明，可穿戴检测系统有潜力实现炎症患者汗液中的低浓度细胞因子监测，以帮助医疗保健系统控制身体的免疫状态。
A wearable sensor capable of directly detecting cytokines in external secretions external to the human body is pivotal for gauging the intensity of the immune response and circumventing inflammatory storms, a significant clinical concern. The detection of low‐concentration cytokines is often hampered due to interference from impurities in secretions. Addressing this challenge, a wearable detection system is developed by integrating a Janus membrane with water auto‐collection/filtering capabilities, and aptamer‐modified graphene field‐effect transistor sensors, enabling the detection of low‐concentration cytokines in undiluted sweat samples. The Janus membrane can automatically filter external secretions and transport them to the sensor surface, effectively eliminating impurity interference and enhancing the sensor's detection capabilities. The device can sensitively detect cytokines (such as TNF‐α, a marker for inflammation and cancer) in undiluted sweat, with a detection range spanning 0.5 to 500 pM, and a detection limit reaching 0.31 pM. The ultra‐flexibility of the sensor is also verified, maintaining stable electrical properties even after enduring up to 50 extreme deformations. These findings indicate that the wearable detection system has the potential to realize low‐concentration cytokines monitoring in the sweat of inflammatory patients, to assist the healthcare system in controlling the body's immune status.