Widespread heteroplasmy in schistosomes makes an mtVNTR marker "nearsighted".
Widespread heteroplasmy in schistosomes makes an mtVNTR marker "nearsighted".
复制标题
血吸虫中广泛的异质性使得 mtVNTR 标记变得“近视”。
DOI：
10.1093/jhered/92.3.248
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Minchella,DJ
中科院分区：
文献类型：
--
作者：
Curtis,J;Fraga,LA;deSouza,CP;Corrêa-Oliveira,R;Minchella,DJ
Mitochondrial markers are often hailed as the preferred DNA elements for analyses of population subdivision. To this end we have employed a mitochondrial repeat element to examine the population structure inSchistosoma mansoni(human blood flukes). Schistosome isolates were collected from each of 21 different patients representing seven different areas of a Brazilian village. These parasite isolates demonstrate substantial genetic polymorphism, with an average of 10 genotypes infecting each patient, which is more readily detected because of high levels of heteroplasmy (i.e., 72.5% of the individual worms exhibit multiple versions of this repeat region with different numbers of repeats). Due to the high number of common haplotypes in the population, this repeat element fromS. mansonihas a large proportion (47%) of its genetic variation described by differences among mitochondrial genomes within individual worms. However, when only rare haplotypes are considered, population structure can be detected. It seems that heteroplasmy in the schistosome population of Melquiades is both the source of plentiful genetic variation and a confounding factor in the analysis of that variation. Thus the schistosome population in Melquiades may actually be more strongly subdivided than we are able to detect using this mitochondrial marker.
