Tissue-specific direct microtransfer of nanomaterials into Drosophila embryos as a versatile in vivo test bed for nanomaterial toxicity assessment.
Tissue-specific direct microtransfer of nanomaterials into Drosophila embryos as a versatile in vivo test bed for nanomaterial toxicity assessment.
复制标题
DOI：
10.2147/ijn.s56459
复制
发表时间：
2014
影响因子：
8
通讯作者：
Carrero-Martínez FA
中科院分区：
文献类型：
--
作者：
Vega-Alvarez S;Herrera A;Rinaldi C;Carrero-Martínez FA
纳米材料是密集的研究主题，集中在其合成，改性和生物医学应用。纳米材料生产的增加及其广泛应用意味着人类和环境接触的风险更高。不幸的是，纳米材料对环境的影响和对生物体的毒性都没有得到充分的了解。需要成本效益高、快速的毒性分析，需要最少量的材料来建立其生物医学潜力和环境安全标准。果蝇通过大量的体内工具和技术证明了一种高效且具有成本效益的模式生物，所有这些工具和技术在其整个生命周期中都具有高通量可扩展性和筛选可行性。在这里，我们报告了通过直接微转移到靶组织中的组织特异性纳米材料评估。我们测试了几种具有潜在生物医学应用的纳米材料，如单壁碳纳米管、多壁碳纳米管、银、金、二氧化钛和氧化铁纳米颗粒。通过评价果蝇发育形态学里程碑的进展来评估纳米材料毒性。这种具有成本效益的评估方法适用于高通量筛选。
Nanomaterials are the subject of intense research, focused on their synthesis, modification, and biomedical applications. Increased nanomaterial production and their wide range of applications imply a higher risk of human and environmental exposure. Unfortunately, neither environmental effects nor toxicity of nanomaterials to organisms are fully understood. Cost-effective, rapid toxicity assays requiring minimal amounts of materials are needed to establish both their biomedical potential and environmental safety standards. Drosophila exemplifies an efficient and cost-effective model organism with a vast repertoire of in vivo tools and techniques, all with high-throughput scalability and screening feasibility throughout its life cycle. Here we report tissue specific nanomaterial assessment through direct microtransfer into target tissues. We tested several nanomaterials with potential biomedical applications such as single-wall carbon nanotubes, multiwall carbon nanotubes, silver, gold, titanium dioxide, and iron oxide nanoparticles. Assessment of nanomaterial toxicity was conducted by evaluating progression through developmental morphological milestones in Drosophila. This cost-effective assessment method is amenable to high-throughput screening.