Towards identification of bioactive compounds in cold vacuum extracted double cherry blossom (Gosen-Sakura) leaves
Towards identification of bioactive compounds in cold vacuum extracted double cherry blossom (Gosen-Sakura) leaves
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鉴定冷真空提取的重瓣樱花（Gosen-Sakura）叶中的生物活性化合物
DOI：
10.1080/15592324.2019.1644594
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Shioda S
中科院分区：
文献类型：
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作者：
Shibato J;Takenoya F;Hirabayashi T;Kimura A;Iwasaki Y;Toyoda Y;Hori H;Tamogami S;Rakwal R;Shioda S
本研究探讨了从双樱花开花（称为“Gosen-Sakura”的Gosen-city，新泻县，日本）的叶子，这已被长期用于制备传统的日本甜（wagashi）-“樱花-mochi”的生物分子化合物的可能性。基于其历史上所显示的抗微生物特性，我们的研究提供了一种新的低温真空提取方法来提取“近天然形式的水溶性叶（细胞）提取物，并通过MTT试验证明了一些”新“化合物的存在，具有抗肿瘤细胞系增殖抑制作用。据我们所知，没有关于樱花树“叶（细胞）提取物”抑制肿瘤细胞系生长的报道。我们进一步检查并比较了具有抗肿瘤活性的已知化合物香豆素和苯甲醇与Gosen-Sakura叶提取物的作用;结果使我们假设Gosen-Sakura叶提取物含有除上述两种已知化合物之外的具有抗肿瘤作用的物质。此外，我们推测通过在DNA合成点靶向细胞分裂并引起细胞凋亡，从而对御前樱花叶提取物的潜在作用机制进行了推测。总之，我们提出了科学证据，证明在长期用于日本甜-'sakura-mochi'的Gosen-Sakura叶中存在某些具有抗肿瘤活性的'新型'生物分子。
The present research examines the possibility of finding bio-molecular compounds from the double cherry blossom (termed as ‘Gosen-Sakura’ of Gosen-city, Niigata-prefecture, Japan) leaves, which have been long used in the preparation of the traditional Japanese sweet (wagashi) – ‘sakura-mochi’. Based on its indicated anti-microbial properties historically, our study provides a new low temperature vacuum extraction method for extracting ‘near natural form of water soluble leaf (cell) extracts from the Gosen-Sakura, and demonstrates the presence of some ‘novel’ compound(s) with anti-tumor cell lines proliferation inhibitory affects through the MTT assay. To our knowledge, no reports exist on the sakura tree ‘leaf (cell) extracts’ inhibiting tumor cell line growth. We further examined and compared the effects of known compounds with anti-tumor activity, coumarin and benzyl alcohol with Gosen-Sakura leaf extract; results lead us to hypothesize that the Gosen-Sakura leaf extract contains substance(s) other than the above two known compounds, with antitumor effect. Additionally, we speculate on the underlying mechanism of action of the Gosen-Sakura leaf extract by targeting cell division at the point of DNA synthesis and causing apoptosis. In conclusion, we present scientific evidence on the presence of certain ‘novel’ biomolecule(s), with anti-tumor activity, in the Gosen-Sakura leaf which has been long used in Japanese sweet – the ‘sakura-mochi’.