ISOLATION AND CHARACTERIZATION OF 15 MICROSATELLITE LOCI IN FOUR ENDANGERED AMENTOTAXUS SPECIES (TAXACEAE)
ISOLATION AND CHARACTERIZATION OF 15 MICROSATELLITE LOCI IN FOUR ENDANGERED AMENTOTAXUS SPECIES (TAXACEAE)
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DOI：
10.3732/ajb.1100452
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发表时间：
2012-04-01
影响因子：
3
通讯作者：
Chiang, Yu-Chung
中科院分区：
文献类型：
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作者：
Ho, Chin-Shang;Chen, Chaur-Tzuhn;Chiang, Yu-Chung
研究前提：在濒危物种台湾穗花杉中开发了 15 个微卫星位点，并在另外 3 个物种（A. argotaenia、A. yunnanensis 和 A. poilanei）中进行了测试，以评估保护工作的种群结构并重建这一古老谱系的系统发育地理模式。等位基因的数量为 2 至 10 个，观察到的杂合度为 0 至 0.60。所有基因座均被发现可在种间扩增。结论：这些多态性和可转移基因座对于未来的研究可能会有用，这些研究将侧重于识别物种内不同的遗传单位，并建立密切相关物种之间的系统发育地理学模式和物种形成过程。
Premise of the study: Fifteen microsatellite loci were developed in an endangered species, Amentotaxus formosana, and were tested in an additional three species, A. argotaenia, A. yunnanensis, and A. poilanei, to evaluate the population structure for conservation efforts and reconstruct the phylogeographic patterns of this ancient lineage.Methods and Results: Polymorphic primer sets were developed from A. formosana; the number of alleles ranged from two to 10, with an observed heterozygosity ranging from 0 to 0.60. All of the loci were found to be interspecifically amplifiable.Conclusions: These polymorphic and transferable loci will be potentially useful for future studies that will focus on identifying distinct genetic units within species and establishing the phylogeographic patterns and the process of speciation among closely related species.