Functional neurogenesis in the adult hippocampus
Functional neurogenesis in the adult hippocampus
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DOI：
10.1038/4151030a
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发表时间：
2002-02-28
期刊：
影响因子：
64.8
通讯作者：
Gage, FH
中科院分区：
文献类型：
--
作者：
van Praag, H;Schinder, AF;Gage, FH
有大量证据表明，成年哺乳动物海马体的齿状回中产生了新的神经元，海马体是大脑中对学习和记忆很重要的区域（1-5）。然而，目前还不知道这些新的神经元是否具有功能，因为用于研究成人神经发生的方法仅限于固定的组织。我们在这里使用了一个逆转录病毒载体，表达只标记分裂细胞的绿色荧光蛋白，并且可以在活体海马切片中观察到。我们报告说，新产生的细胞在成年小鼠海马神经元的形态，可以显示被动膜特性，动作电位和功能性突触输入类似于成熟的齿状颗粒细胞中发现的。我们的研究结果表明，在成年哺乳动物大脑中，新产生的细胞成熟为功能性神经元。
There is extensive evidence indicating that new neurons are generated in the dentate gyrus of the adult mammalian hippocampus, a region of the brain that is important for learning and memory(1-5). However, it is not known whether these new neurons become functional, as the methods used to study adult neurogenesis are limited to fixed tissue. We use here a retroviral vector expressing green fluorescent protein that only labels dividing cells, and that can be visualized in live hippocampal slices. We report that newly generated cells in the adult mouse hippocampus have neuronal morphology and can display passive membrane properties, action potentials and functional synaptic inputs similar to those found in mature dentate granule cells. Our findings demonstrate that newly generated cells mature into functional neurons in the adult mammalian brain.