ENHANCEMENT OF THE GENERATION OF CYTOTOXIC T-CELLS BY ENDOGENOUS OPIATES
ENHANCEMENT OF THE GENERATION OF CYTOTOXIC T-CELLS BY ENDOGENOUS OPIATES
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DOI：
10.1016/0165-5728(86)90099-8
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发表时间：
1986-07-01
影响因子：
3.3
通讯作者：
KLIMPEL, GR
中科院分区：
文献类型：
--
作者：
CARR, DJJ;KLIMPEL, GR
先前的报道已经表明内源性阿片剂（β-内啡肽和甲硫氨酸脑啡肽）在生理浓度下对免疫系统的细胞具有作用。使用鼠脾细胞，我们在此报道了β-内啡肽和甲硫氨酸脑啡肽可以在单向混合淋巴细胞培养（MLC）中在同种异体抗原的次优浓度下增强细胞毒性T淋巴细胞（CTL）的产生。这种增强在2.9 × 10 - 4的水平范围内可见。10-8 M至2.9 ×10-14 M和1.45 ×10-6 M至1.45 ×10-12 M用于β-内啡肽和甲硫氨酸脑啡肽。这种增强作用可被10-7 M的纳洛酮部分阻断。此外，同时加入甲硫氨酸-脑啡肽和β-甲硫氨酸-脑啡肽也是可行的。内啡肽不增加单独的阿片肽对CTL产生的增强作用，这表明它们通过相同的受体起作用。这份报告为神经内分泌系统和免疫系统之间的调节回路理论提供了额外的证据。
Previous reports have shown that endogenous opiates (.beta.-endorphin and met-enkephalin) have effects on cells of the immune system at physiologic concentrations. Using murine spleen cells, we herein report that .beta.-endorphin and met-enkephalin can enhance the generation of cytotoxic T lymphocytes (CTLs) at suboptimal concentrations of alloantigen in a one-way mixed lymphocyte culture (MLC). This enhancement is seen at levels ranging from 2.9 .times. 10-8 M to 2.9 .times. 10-14 M and 1.45 .times. 10-6 M to 1.45 .times. 10-12 M for .beta.-endorphin and met-enkephalin, respectively. This enhancement can be partially blocked by naloxone at 10-7 M. Furthermore, the simultaneous addition of met-enkephalin and .beta.-endorphin does not increase the enhancement of CTL generation by either opiate peptide alone suggesting that they are acting through the same receptor. This report gives added proof for the regulatory-loop theory between the neuroendocrine and immune systems.