Selective inhibition of carotenoid cleavage dioxygenases: phenotypic effects on shoot branching.
Selective inhibition of carotenoid cleavage dioxygenases: phenotypic effects on shoot branching.
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类胡萝卜素裂解双加氧酶的选择性抑制：对枝条分枝的表型影响。
DOI：
10.1074/jbc.m805453200
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发表时间：
2009-02-20
期刊：
影响因子：
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通讯作者：
Thompson AJ
中科院分区：
文献类型：
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作者：
Sergeant MJ;Li JJ;Fox C;Brookbank N;Rea D;Bugg TD;Thompson AJ
类胡萝卜素裂解双加氧酶家族的成员催化类胡萝卜素在各种链位置的氧化裂解，导致形成广泛的脱辅基类胡萝卜素信号分子。为了探索这个不同的酶家族的功能，我们已经使用了化学遗传学的方法来设计不同类别的类胡萝卜素裂解双加氧酶的选择性抑制剂。合成了一组18个芳烷基-异羟肟酸，其中铁螯合异羟肟酸和芳环之间的距离是不同的;这些化合物被筛选为四种不同酶类的抑制剂，无论是在体外还是在体内。有效的抑制剂被发现，选择性地抑制酶，裂解类胡萝卜素在9，10位，50%的抑制，实现了在亚微摩尔浓度。在100 μm处将某些抑制剂应用于拟南芥节外植体或整株植物导致增加的芽分支，与9，10-切割的抑制一致。
Members of the carotenoid cleavage dioxygenase family catalyze the oxidative cleavage of carotenoids at various chain positions, leading to the formation of a wide range of apocarotenoid signaling molecules. To explore the functions of this diverse enzyme family, we have used a chemical genetic approach to design selective inhibitors for different classes of carotenoid cleavage dioxygenase. A set of 18 arylalkyl-hydroxamic acids was synthesized in which the distance between an iron-chelating hydroxamic acid and an aromatic ring was varied; these compounds were screened as inhibitors of four different enzyme classes, either in vitro or in vivo. Potent inhibitors were found that selectively inhibited enzymes that cleave carotenoids at the 9,10 position; 50% inhibition was achieved at submicromolar concentrations. Application of certain inhibitors at 100 μm to Arabidopsis node explants or whole plants led to increased shoot branching, consistent with inhibition of 9,10-cleavage.