Continuous ultrafiltration/diafiltration using a 3D‐printed two membrane single pass module
Continuous ultrafiltration/diafiltration using a 3D‐printed two membrane single pass module
复制标题
使用 3D 打印双膜单通道模块进行连续超滤/渗滤
DOI：
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发表时间：
2019
影响因子：
3.8
通讯作者：
M. Franzreb
中科院分区：
文献类型：
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作者：
Ruijie Tan;M. Franzreb
3D打印的超滤/渗滤（UF/DF）模块允许连续、同时浓缩保留的（生物）分子并减少或交换盐缓冲液。与文献中已知的单程UF概念不同，DF操作不需要应用几个步骤或中间稀释单元。相比之下，开发的模块使用两个膜限制部分，其中分子被集中，而样品通过。在该浓缩过程的同时，两个膜允许DF缓冲液垂直流入和流出，从而降低该部分中的盐含量。该模块显示出溶解蛋白质的连续浓度高达4.6倍，同时沿着仅5 cm的流动路径长度将盐浓度降低至初始浓度的47%沿着。由于单程操作，该模块显示出浓差极化效应，在较高浓缩因子的情况下，会降低所用膜的有效渗透率。然而，由于其简单的设计和能力，同时运行UF和DF过程中的一个单一的模块，开发可能是经济上有益的小规模UF/DF应用。
A 3D printed ultrafiltration/diafiltration (UF/DF) module is presented allowing the continuous, simultaneous concentration of retained (bio‐)molecules and reduction or exchange of the salt buffer. Differing from the single‐pass UF concepts known from the literature, DF operation does not require the application of several steps or units with intermediating dilution. In contrast, the developed module uses two membranes confining the section in which the molecules are concentrated while the sample is passing. Simultaneously to this concentration process, the two membranes allow a perpendicular in and outflow of DF buffer reducing the salt content in this section. The module showed the continuous concentration of a dissolved protein up to a factor of 4.6 while reducing the salt concentration down to 47% of the initial concentration along a flow path length of only 5 cm. Due to single‐pass operation the module shows concentration polarization effects reducing the effective permeability of the applied membrane in case of higher concentration factors. However, because of its simple design and the capability to simultaneously run UF and DF processes in a single module, the development could be economically beneficial for small scale UF/DF applications.