A FRET-based aptasensor for ochratoxin A detection using graphitic carbon nitride quantum dots and CoOOH nanosheets as donor-acceptor pair
A FRET-based aptasensor for ochratoxin A detection using graphitic carbon nitride quantum dots and CoOOH nanosheets as donor-acceptor pair
复制标题
基于 FRET 的适体传感器，使用石墨氮化碳量子点和 CoOOH 纳米片作为供体-受体对检测赭曲霉毒素 A
DOI：
10.1016/j.talanta.2020.121159
复制
发表时间：
2020-10-01
期刊：
影响因子：
6.1
通讯作者：
You, Tianyan
中科院分区：
文献类型：
--
作者：
Bi, Xiaoya;Luo, Lijun;You, Tianyan
由于羟基氧化钴（CoOOH）纳米片良好的猝灭功能，开发了一种用于玉米和大麦粉中赭曲霉毒素A（OTA）分析的新型荧光适体传感器。首次报道了石墨氮化碳量子点（g-CNQDs）和CoOOH纳米片作为高效能量供体-受体对的组合，并通过研究g-CNQDs的荧光寿命证明了猝灭机制。适配体修饰的 g-CNQDs (g-CNQDs-apt) 通过范德华力吸附到 CoOOH 纳米片表面。因此，从 g-CNQDs-apt 到 CoOOH 纳米片的福斯特共振能量转移 (FRET) 被启动，导致荧光猝灭。添加OTA后，线性适配体与OTA特异性结合，形成G-四链体，与CoOOH纳米片的相互作用相对较弱，并从纳米片表面分离。因此，g-CNQDs-apt和CoOOH纳米片之间的FRET过程受到阻碍，导致g-CNQDs-apt的荧光明显恢复。开发的适体传感器表现出可接受的检测限（0.5 nM）和从 1 nM 到 140 nM 的理想线性关系。同时，适配体传感器具有操作简便、检测快速、选择性高等优点。此外，适配体传感平台也适用于谷物（大麦和玉米粉）中的OTA测定，回收率在94.5%至101%之间，相对标准偏差在2.24%以下。
A new fluorescence aptasensor for Ochratoxin A (OTA) analysis in corn and barley flour was developed owing to the favourable quenching function of cobalt oxyhydroxide (CoOOH) nanosheets. The first combination of graphitic carbon nitride quantum dots (g-CNQDs) and CoOOH nanosheets as efficient energy donor-acceptor pair was reported, and the quenching mechanism was proved by investigating the fluorescence lifetime of g-CNQDs. The aptamer-modified g-CNQDs (g-CNQDs-apt) were adsorbed onto CoOOH nanosheets surface by van der Waals force. Consequently, the Forster resonance energy transfer (FRET) from g-CNQDs-apt to CoOOH nanosheets was initiated, leading to quenched fluorescence. With the addition of OTA, the linear aptamer specifically bound with OTA to form G-quadruplex, which had relatively weak interaction with the CoOOH nanosheets and separated from the nanosheets surface. Thus, the FRET process between g-CNQDs-apt and CoOOH nanosheets was hindered, leading to the fluorescence of g-CNQDs-apt recovered clearly. The developed aptasensor exhibited acceptable detection limit with 0.5 nM and desirable linear relationship from 1 nM to 140 nM. Meanwhile, the aptasensor possessed multiple advantages, including easy operation, rapid detection and high selectivity. Moreover, the aptamer sensing platform was favorably applied for OTA determination in cereal (barley and corn flour), in which the recoveries varied from 94.5% to 101% with the relative standard deviation under 2.24%.