ENZYME COLLAGEN MEMBRANE FOR ELECTROCHEMICAL DETERMINATION OF GLUCOSE
ENZYME COLLAGEN MEMBRANE FOR ELECTROCHEMICAL DETERMINATION OF GLUCOSE
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用于电化学测定葡萄糖的酶胶原膜
DOI：
10.1021/ac50037a031
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发表时间：
1979
影响因子：
7.4
通讯作者：
J. Laurent
中科院分区：
文献类型：
--
作者：
R. Sternberg;P. Coulet;J. Laurent
利用电化学传感器设计了一种新型微量葡萄糖分析仪。该差示装置包括：(A)葡萄糖传感器，由修饰的气体电极和多孔膜组成，其中pH检测器被铂盘取代，多孔膜由胶原膜组成，在胶原膜上经酰基-叠氮活化处理后，β-D-葡萄糖氧化酶已共价结合；(B)补偿电极，其上安装有非酶胶原膜。在将含有葡萄糖的样品注入反应容器中后，在其中浸泡探针，在酶工作电极处检测到阳极电流。对两个电极的电流输出进行相减和二次微分；达到稳态，并记录静态和动态响应。在0.1µM-2 mm范围内，这两种响应都与葡萄糖浓度成正比，在这些条件下，重复性好于2%。我们的系统表现出极高的灵敏度，即10 nm，比以前报道的数据高出3个数量级，非常适合食品和生物样品中微量葡萄糖的分析。
A new trace glucose analyzer has been designed using electrochemical sensors. The differential device includes (a) a glucose sensor consisting of a modified gas electrode in which ihe pH detector was replaced by a platinum disk and the porous film by a collagen membrane on which beta-D-glucose oxidase has been covalently bound after an acyl-azide activation process; (b) a compensating electrode mounted with a nonenzymatic collagen membrane. After injection of a glucose containing sample into the reaction vessel, where the probes are immersed, an anodic current is detected at the enzyme working electrode. Current outputs of both electrodes are subtracted and twice differentiated; a steady state is reached and the stationary and dynamic responses are recorded. Both responses are proportional to glucose concentration in the 0.1 µM-2 mM range, and the reproducibility was found to be better than 2% using these conditions. The extreme sensitivity exhibited by our system, i.e., 10 nM, is better than previously reported data by 3 orders of magnitude, and is very favorable for trace glucose assays in food and biological samples.