Bacillus amyloliquefaciens LZ04 improves the resistance of Arabidopsis thaliana to high calcium stress and the potential role of lncRNA-miRNA-mRNA regulatory network in the resistance
Bacillus amyloliquefaciens LZ04 improves the resistance of Arabidopsis thaliana to high calcium stress and the potential role of lncRNA-miRNA-mRNA regulatory network in the resistance
复制标题
解淀粉芽孢杆菌LZ04提高拟南芥对高钙胁迫的抗性及lncRNA-miRNA-mRNA调控网络在抗性中的潜在作用
DOI：
10.1016/j.plaphy.2020.03.022
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发表时间：
2020
影响因子：
6.5
通讯作者：
Zhang Jinlin
中科院分区：
文献类型：
--
作者：
Li Fei;Shi Tianlong;He Aolei;Huang Xiaolong;Gong Jiyi;Yi Yin;Zhang Jinlin
淀粉解性芽孢杆菌是一种非致病、促进植物生长的根际细菌，能增强植物对干旱和疾病的抗性。拟南芥是探索微生物-植物相互作用的多用途模式植物，也是分子研究的重要植物工具。非编码RNA是参与调节多种生物学功能的RNA分子，是植物生物学领域的研究热点。在本研究中，B的作用。淀粉酶对金黄色葡萄球菌抗性的影响。对海棠高钙胁迫进行了分析。转录组测序。在4种处理条件下进行海燕草根培养，筛选差异表达的lncRNAs、mRNAs和miRNAs。并进行功能分析，以了解B。溶淀粉菌调控的lncRNAs、miRNAs和mRNAs影响抗性。塔里亚诺高钙胁迫。结果表明，B。淀粉溶液处理增加了对赤霉病的抗性。塔里亚诺高钙胁迫。筛选出一组高钙组和对照组、高钙组和高钙+淀粉液化杆菌组之间的差异表达的lncRNAs、mRNAs和miRNAs。功能分析表明，差异表达的mRNAs和miRNA参与多种生物学功能，高钙胁迫引起的转录失调涉及代谢过程而不是防御反应。综上所述，解淀粉芽孢杆菌可提高对金黄色葡萄球菌的抗性。Talianato通过lncRNA-miRNA-mRNA调控网络实现高钙胁迫。这些研究结果将有助于高钙岩溶地区农业的发展。
Bacillus amyloliquefaciens is a non-pathogenic and plant growth-promoting rhizobacterium that enhances plant resistance to drought and diseases. Arabidopsis thaliana is a multipurpose model plant for exploring microorganism-plant interactions and a crucial vegetal tool for molecular research. Non-coding RNAs are RNA molecules involved in the regulation of various biological functions and constitute a research hotspot in the field of plant biology. In this study, the effect ofB. amyloliquefacienstreatment on the resistance ofA. thalianato high calcium stress was analyzed. The transcriptome sequencing ofA. thalianaroots under four treatment conditions was performed to screen differentially expressed lncRNAs, mRNAs and miRNAs. Functional analysis was also performed to understand the potential mechanism by whichB. amyloliquefaciens-regulated lncRNAs, miRNAs and mRNAs affect the resistance ofA. thalianato high calcium stress. The results indicated thatB. amyloliquefacienstreatment increased the resistance ofA. thalianato high calcium stress. A set of differentially expressed lncRNAs, mRNAs and miRNAs were screened between the high calcium and control group on one hand, and high calcium and high calcium +B. amyloliquefaciensgroups on the other hand. Functional analysis indicated that the differentially expressed mRNAs and miRNA were involved in various biological functions and that transcriptional dysregulation caused by high calcium stress involves metabolic processes rather than defense responses. Conclusively,B. amyloliquefaciensmay improve the resistance ofA. thalianato high calcium stress via a lncRNA-miRNA-mRNA regulatory network. These findings will contribute to the development of agriculture in karst regions with high calcium content.