Stressor Exposure Disrupts Commensal Microbial Populations in the Intestines and Leads to Increased Colonization by Citrobacter rodentium
Stressor Exposure Disrupts Commensal Microbial Populations in the Intestines and Leads to Increased Colonization by Citrobacter rodentium
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DOI：
10.1128/iai.00862-09
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发表时间：
2010-04-01
影响因子：
3.1
通讯作者：
Lyte, Mark
中科院分区：
文献类型：
--
作者：
Bailey, Michael T.;Dowd, Scot E.;Lyte, Mark
胃肠道中栖息着大量微生物，已知这些微生物对宿主有许多有益作用。先前的研究表明，压力源暴露会破坏肠道微生物群的稳定性，但这些变化的程度以及对肠道感染的影响尚未得到很好的表征。为了检查压力源诱导肠道微生物群变化的能力，我们将小鼠暴露于长期束缚压力源下，然后使用传统培养技术和细菌标签编码的 FLX 扩增子焦磷酸测序 (bTEFAP) 来表征肠道中的微生物种群。暴露于应激源会导致兼性厌氧微生物群过度生长，同时显着降低应激小鼠盲肠中的微生物丰富度和多样性。其中一些影响可以通过压力源引起的紫单胞菌科细菌相对丰度的减少来解释。为了确定这些改变是否会导致病原体定植增加，对应激小鼠以及未应激小鼠进行口服肠道鼠病原体啮齿类柠檬酸杆菌的攻击。与非应激对照小鼠相比，暴露于束缚应激源导致啮齿类柠檬酸杆菌定植显着增加。定植增加与结肠组织中肿瘤坏死因子α（TNF-α）基因表达增加相关。总之，这些数据表明，长期的压力源可以显着改变肠道微生物群的组成，并表明微生物群的这种破坏增加了对肠道病原体的易感性。
The gastrointestinal tract is colonized by an enormous array of microbes that are known to have many beneficial effects on the host. Previous studies have indicated that stressor exposure can disrupt the stability of the intestinal microbiota, but the extent of these changes, as well as the effects on enteric infection, has not been well characterized. In order to examine the ability of stressors to induce changes in the gut microbiota, we exposed mice to a prolonged restraint stressor and then characterized microbial populations in the intestines using both traditional culture techniques and bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP). Exposure to the stressor led to an overgrowth of facultatively anaerobic microbiota while at the same time significantly reducing microbial richness and diversity in the ceca of stressed mice. Some of these effects could be explained by a stressor-induced reduction in the relative abundance of bacteria in the family Porphyromonadaceae. To determine whether these alterations would lead to increased pathogen colonization, stressed mice, as well as nonstressed controls, were challenged orally with the enteric murine pathogen Citrobacter rodentium. Exposure to the restraint stressor led to a significant increase in C. rodentium colonization over that in nonstressed control mice. The increased colonization was associated with increased tumor necrosis factor alpha (TNF-alpha) gene expression in colonic tissue. Together, these data demonstrate that a prolonged stressor can significantly change the composition of the intestinal microbiota and suggest that this disruption of the microbiota increases susceptibility to an enteric pathogen.