Toxicity Assessment of the Xanthid Crab Demania cultripes from Cebu Island, Philippines.
Toxicity Assessment of the Xanthid Crab Demania cultripes from Cebu Island, Philippines.
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DOI：
10.1155/2010/172367
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Shindo, Jo
中科院分区：
文献类型：
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作者：
Asakawa, Manabu;Gomez-Delan, Gloria;Shindo, Jo
印度-太平洋地区报告了多起因摄入珊瑚礁蟹而导致人员死亡的中毒案件。我们从食品卫生的角度评估了从菲律宾中部米沙鄢地区宿务岛东海岸附近的卡莫特斯海采集的不明黄蟹的毒性。 2006 年采集的所有 7 个标本均被鉴定为邪疯狂，无论采集季节如何，都对小鼠有毒，并会诱发典型的河豚毒素 (TTX) 和麻痹性贝类毒素 (PSP) 麻痹症状。活性以小鼠单位(MU)表示，定义为腹膜内注射后30分钟内杀死20g ddY雄性小鼠的TTX量。注射。标本内脏和附肢的毒性评分分别为 18.2 ± 16.0（平均值 ± S.D.）和 4.4 ± 2.6MU/g。内脏和附肢观察到的最高个体毒性评分分别为 52.1 和 7.7MU/g。有毒样品出现频率为100%。通过高效液相色谱-荧光检测分析 (HPLC-FLD) 测定的毒素谱显示，TTX 是主要毒性成分，与 4-epi TTX 和 4,9-anHydroTTX 一起约占总毒性的 90%。此外，气相色谱-质谱 (GC-MS) 分析显示质量碎片离子峰位于 m/z 376、392 和 407，这是 TTX 特有的喹唑啉骨架（C9 碱基）的特征。此外，仅检测到少量含有膝沟藻毒素1-4和羟基石房蛤毒素的PSP。据我们所知，这是第一份提供 TTX 和 PSP 在宿雾岛周围水域的黄蟹 D. cultripes 中出现的证据的报告。从食品卫生的角度来看，应警告沿海地区的人们这种螃蟹的潜在危害，以防止有意或无意食用。
Several cases of poisoning resulting in human fatalities and stemming from the ingestion of coral reef crabs have been reported from the Indo-Pacific region. We assessed the toxicity of the unidentified xanthid crab collected from the Camotes Sea off the eastern coast of Cebu Island, central Visayas region of Philippines from the food hygienic point of view. All seven specimens, which were identified with Demania cultripes, collected in 2006 were toxic to mice irrespective of the season of collection and induced paralytic symptoms typical of tetrodotoxin (TTX) and paralytic shellfish poison (PSP). The activity was expressed in mouse unit (MU) being defined as the amount of TTX to kill a 20g ddY male mice in 30min after i.p. injection. Toxicity scores for viscera and appendages of specimens were 18.2 ± 16.0 (mean ± S.D.) and 4.4 ± 2.6MU/g, respectively. The highest individual toxicity scores observed for viscera and appendages were 52.1 and 7.7MU/g, respectively. The frequency of toxic samples was 100%. Toxin profiles as determined by high-performance liquid chromatography-fluorescent detection analysis (HPLC-FLD) revealed that TTX was the main toxic principle accounting for about 90% of the total toxicity along with 4-epi TTX and 4,9-anhydroTTX. Furthermore, gas chromatography-mass spectrometry (GC-MS) analysis revealed mass fragment ion peaks at m/z 376, 392 and 407, which were characteristic of the quinazoline skeleton (C9-base) specific to TTX. In addition, only a small amount of PSP containing gonyautoxins1-4 and hydroxysaxitoxin was detected. To our knowledge, this is the first report presenting evidence of occurrence of TTX and PSP in the xanthid crab D. cultripes inhabiting waters surrounding Cebu Island. From food hygienic point of view, people in coastal areas should be warned of the potential hazard of this crab in order to prevent its intentional or accidental consumption.