Transplantation of Neural Stem Cells Cultured in Alginate Scaffold for Spinal Cord Injury in Rats.
Transplantation of Neural Stem Cells Cultured in Alginate Scaffold for Spinal Cord Injury in Rats.
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DOI：
10.4184/asj.2016.10.4.611
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发表时间：
2016-08
影响因子：
2.3
通讯作者：
Semsar-Kazerooni M
中科院分区：
文献类型：
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作者：
Hosseini SM;Sharafkhah A;Koohi-Hosseinabadi O;Semsar-Kazerooni M
本研究调查了海藻酸盐封装神经干细胞（NSC）移植对 Sprague-Dawley 雄性大鼠脊髓损伤的影响。移植后 6 周评估神经功能，并进行组织学研究和 caspase-3 水平测量。本研究的目的是探索藻酸盐移植中培养的神经干细胞是否可以改善脊髓损伤的恢复。脊髓损伤是导致残疾的主要原因之一，并且没有有效的治疗方法。脊髓损伤也会导致感觉障碍。随着在各种中枢神经系统环境中使用干细胞疗法的推动，人们对使用干细胞治疗脊髓损伤产生了兴趣。神经干细胞是一种能够分化为所有三种神经谱系的干细胞，在脊髓损伤治疗中显示出前景。此外，许多研究表明，在海藻酸盐等三维 (3D) 支架中培养 NSC 可以增强神经分化。 NSCs 是从 14 天大的大鼠胚胎中分离出来的。分离的 NSC 在含有碱性成纤维细胞生长因子和内皮生长因子的生长培养基中培养。这些细胞的特征是分化为三种神经谱系，并将它们培养在藻酸盐支架中。 7天后，细胞被封装并移植到脊髓损伤的大鼠模型中。我们的数据表明，在藻酸盐 3D 支架中培养和 NSC 移植可以改善脊髓损伤大鼠模型的神经系统结果。在封装神经干细胞移植病例中，炎症评分、病变大小以及 caspase-3 活性（用于细胞凋亡评估）均较低。在藻酸盐支架中培养的 NSC 移植在大鼠模型中脊髓损伤恢复方面取得了更好的临床和组织学结果。
This study investigated the effects of transplantation of alginate encapsulated neural stem cells (NSCs) on spinal cord injury in Sprague-Dawley male rats. The neurological functions were assessed for 6 weeks after transplantation along with a histological study and measurement of caspase-3 levels. The aim of this study was to discover whether NSCs cultured in alginate transplantation improve recovery from spinal cord injury. Spinal cord injury is one of the leading causes of disability and it has no effective treatment. Spinal cord injury can also cause sensory impairment. With an impetus on using stem cells therapy in various central nervous system settings, there is an interest in using stem cells for addressing spinal cord injury. Neural stem cell is one type of stem cells that is able to differentiate to all three neural lineages and it shows promise in spinal injury treatment. Furthermore, a number of studies have shown that culturing NSCs in three-dimensional (3D) scaffolds like alginate could enhance neural differentiation. The NSCs were isolated from 14-day-old rat embryos. The isolated NSCs were cultured in growth media containing basic fibroblast growth factor and endothelial growth factor. The cells were characterized by differentiating to three neural lineages and they were cultured in an alginate scaffold. After 7 days the cells were encapsulated and transplanted in a rat model of spinal cord injury. Our data showed that culturing in an alginate 3D scaffold and transplantation of the NSCs could improve neurological outcome in a rat model of spinal cord injury. The inflammation scores and lesion sizes and also the activity of caspase-3 (for apoptosis evaluation) were less in encapsulated neural stem cell transplantation cases. Transplantation of NSCs that were cultured in an alginate scaffold led to a better clinical and histological outcome for recovery from spinal cord injury in a rat model.