Transformation of Cellular Factor XIII into an Active Zymogen Transglutaminase in Thrombin-Stimulated Platelets
Transformation of Cellular Factor XIII into an Active Zymogen Transglutaminase in Thrombin-Stimulated Platelets
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凝血酶刺激的血小板中细胞因子 XIII 转化为活性酶原转谷氨酰胺酶
DOI：
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发表时间：
1995
影响因子：
6.7
通讯作者：
J. Polgár
中科院分区：
文献类型：
--
作者：
L. Muszbek;G. Haramura;J. Polgár
血液凝固因子XIII（FXIII）的细胞形式存在于血小板、单核细胞和巨噬细胞中。在凝血酶对血小板的长期刺激过程中，细胞FXIII被激活并交联蛋白质，然而，其激活机制尚未阐明。结果表明，与血浆FXIII相反，血小板FXIII的细胞内活化不涉及蛋白水解。FXIII在凝血酶活化的血小板中保持完整，即，活化肽没有从分子中除去。部分酶原FXIII分子，然而，假定一个活跃的配置，这是证明了转氨酶活性的测量和活性位点-SH滴定。这些发现清楚地表明，在血小板活化过程中，当细胞内Ca 2+浓度升高时，发生缓慢的FXIII酶原非蛋白水解转化为活性转氨酶。
Summary The cellular form of blood coagulation factor XIII (FXIII) is present in platelets, monocytes and macrophages. During long-term stimulation of platelets by thrombin cellular FXIII becomes activated and crosslinks proteins, however, the mechanism of its activation has not been elucidated. It was shown that, contrary to plasma FXIII, the intracellular activation of platelet FXIII does not involve proteolysis. FXIII remained intact in thrombin-activated platelets, i.e., the activation peptide was not removed from the molecule. Part of the zymogen FXIII molecules, however, assumed an active configuration as was demonstrated both by the measurement of transglutaminase activity and by active-site-SH titration. These findings clearly indicate that during platelet activation, when intracellular Ca2+ concentration is raised, a slow non-proteolytic transformation of FXIII zymogen into an active transglutaminase occurs.
人血小板中的糖蛋白 Ib 和糖蛋白 IX 通过硫酯键被棕榈酸酰化。
DOI：
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发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Muszbek,L;Laposata,M
通讯作者：
Laposata,M