EVALUATION OF RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM IN CUCUMIS-MELO
EVALUATION OF RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM IN CUCUMIS-MELO
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DOI：
10.1007/bf00224286
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发表时间：
1992-01-01
影响因子：
5.4
通讯作者：
NEUHAUSEN, SL
中科院分区：
文献类型：
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作者：
NEUHAUSEN, SL
本研究的目的是评估甜瓜限制性片段长度多态性（RFLP）的程度，并确定栽培品种之间的相互关系。基因组PstI文库的初步筛选显示，大约40%的克隆是重复的。将162个独特的低拷贝序列无性系与7个甜瓜品种和一个甜瓜品种“Pacer”杂交，以评价RFLP的变化。其中，130个（80%）探针检测到甜瓜与sativus之间的多态性，并且大多数探针具有多个酶消化的多态性。相比之下，只有53个探针（33%）可用于区分至少一个7个接入。其中，只有9%具有不止一种酶消化的信息。这表明，在甜瓜内，不同种质间的差异主要是由于不频繁的碱基替换，而两个物种之间的差异主要是由于基因组的重排，如插入和缺失或大量的碱基替换。其中34个探针用于分析44个甜瓜系，建立RFLP杂交图谱数据库。基于RFLP配置文件计算线之间的相似性百分比，并通过主成分分析进行分析，以可视化线之间的关系。甜瓜和蜜瓜种群之间有明确的界限，但内部没有。
The objectives of this study were to assess the degree of restriction fragment length polymorphism (RFLP) in Cucumis melo and to determine interrelationships among cultivated varieties. Initial screening of a genomic PstI library revealed that approximately 40% of the clones were repetitive. A total of 162 unique and low-copy sequence clones were hybridized to seven diverse accessions of C. melo and a C. sativus cultivar 'Pacer' to evaluate RFLP variation. Of these, 130 probes (80%) detected a polymorphism between C. melo accessions and C. sativus, and the majority were polymorphic with more than one enzyme digest. In contrast, only 53 probes (33%) were useful in differentiating at least one of the seven accessions. Of those, only 9% were informative with more than one enzyme digest. This indicates that within C. melo, the differences among accessions are due to infrequent base substitutions, whereas between the two species, differences are mainly due to genome rearrangements such as insertions and deletions or numerous base substitutions. Of the informative probes, 34 were used in analyzing 44 C. melo lines to establish a data base of RFLP hybridization patterns. Percent similarity based on RFLP profiles was computed among lines and analyzed by principal component analysis, to visualize relationships among lines. There were clear demarcations among, but not within, muskmelon and honeydew groups.