Identification and receptor mechanism of TIR-catalyzed small molecules in plant immunity
Identification and receptor mechanism of TIR-catalyzed small molecules in plant immunity
复制标题
DOI：
10.1126/science.abq3297
复制
发表时间：
2022-07-29
期刊：
影响因子：
56.9
通讯作者：
Chai, Jijie
中科院分区：
文献类型：
--
作者：
Huang, Shijia;Jia, Aolin;Chai, Jijie
具有N-末端Toll/白介素-1受体（TIR）结构域正义病原体效应物的植物核苷酸结合富含亮氨酸重复序列（NLR）受体，以使TIR编码的烟酰胺腺嘌呤二核苷酸水解酶（NAD酶）活性能够用于免疫信号传导。TIR-NLR信号传导需要辅助NLR N需求基因1（NRG 1）、活化的疾病抗性1（ADR 1）和增强的疾病易感性1（EDS 1），其与其旁系同源物植物抗毒素缺陷4（PAD 4）和衰老相关基因101（SAG 101）中的每一个形成异二聚体。在这里，我们表明，含TIR的蛋白质在体外和植物中催化2 '-（5“-磷酸核糖基）-5'-腺苷一磷酸（pRib-AMP）和二磷酸（pRib-ADP）的产生。生化和结构数据表明，EDS 1-PAD 4是pRib-AMP和pRib-ADP的受体复合物，其变构促进EDS 1-PAD 4与ADR 1-L1而不是NRG 1A的相互作用。我们的研究鉴定了TIR催化的pRib-AMP和pRib-ADP作为TIR信号通过EDS 1-PAD 4的缺失环节，并且作为植物免疫的可能的第二信使。
Plant nucleotide-binding leucine-rich repeat-containing (NLR) receptors with an N-terminal Toll/ interleukin-1 receptor (TIR) domain sense pathogen effectors to enable TIR-encoded nicotinamide adenine dinucleotide hydrolase (NADase) activity for immune signaling. TIR-NLR signaling requires the helper NLRs N requirement gene 1 (NRG1), Activated Disease Resistance 1 (ADR1), and Enhanced Disease Susceptibility 1 (EDS1), which forms a heterodimer with each of its paralogs Phytoalexin Deficient 4 (PAD4) and Senescence-Associated Gene 101 (SAG101). Here, we show that TIR-containing proteins catalyze the production of 2'-(5''-phosphoribosyl)-5'-adenosine monophosphate (pRib-AMP) and diphosphate (pRib-ADP) in vitro and in planta. Biochemical and structural data demonstrate that EDS1PAD4 is a receptor complex for pRib-AMP and pRib-ADP, which allosterically promote EDS1-PAD4 interaction with ADR1-L1 but not NRG1A. Our study identifies TIR-catalyzed pRib-AMP and pRib-ADP as a missing link in TIR signaling through EDS1-PAD4 and as likely second messengers for plant immunity.