MOST INFLUENZA-A VIRUS-SPECIFIC MEMORY CYTO-TOXIC LYMPHOCYTES-T REACT WITH ANTIGENIC EPITOPES ASSOCIATED WITH INTERNAL VIRUS DETERMINANTS
MOST INFLUENZA-A VIRUS-SPECIFIC MEMORY CYTO-TOXIC LYMPHOCYTES-T REACT WITH ANTIGENIC EPITOPES ASSOCIATED WITH INTERNAL VIRUS DETERMINANTS
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DOI：
10.1084/jem.159.2.365
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发表时间：
1984-01-01
影响因子：
15.3
通讯作者：
KRAMMER, PH
中科院分区：
文献类型：
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作者：
KEES, U;KRAMMER, PH
在有限稀释中测试的大多数小鼠 (C57BL/6) 甲型流感病毒特异性记忆细胞毒性 T 淋巴细胞 (CTL) 克隆不与甲型流感病毒表面糖蛋白、血凝素 (HA) 和神经氨酸酶 (NA) 发生反应。感染甲型流感病毒株 Aichi (H3N2)、PR8 (H1N1) 或重组株 X31 (H3N2) 的同基因靶细胞的裂解表明，大多数识别的抗原表位与内部病毒决定簇相关。 X31 和 PR8 共享内部病毒决定因素，X31 和 Aichi 共享外部病毒决定因素。在用携带与引发病毒同源的内部决定簇的病毒感染的靶细胞的实验中观察到广泛的CTL交叉反应性。当引发病毒和用于感染靶细胞的病毒之间的内部病毒决定簇不同时，尽管HA和NA是同源的，但发现引发病毒几乎具有完全的CTL特异性。甲型流感病毒特异性 CTL 的主要反应性不同于抗甲型流感抗体，后者主要针对病毒表面糖蛋白上的表位。这一发现可能与甲型流感病毒特异性 CTL 在不同甲型流感病毒反复感染中的作用有关。
Most murine (C57BL/6) influenza A virus-specific memory cytotoxic T lymphocyte (CTL) clones tested in limiting dilution did not react with the influenza A virus surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA). The lysis of syngeneic target cells infected with the influenza A virus strains, Aichi (H3N2), PR8 (H1N1) or recombinant strain X31 (H3N2) indicates that most antigenic epitopes recognized are associated with internal virus determinants. X31 and PR8 share the internal, and X31 and Aichi the external, viral determinants. Extensive CTL cross-reactivity was observed in experiments with target cells infected with virus carrying internal determinants homologous with the priming virus. When the internal viral determinants differed between the priming virus and the virus used to infect the target cells, and although HA and NA were homologous, almost complete CTL specificity for the priming virus was found. The predominant reactivity of influenza A virus-specific CTL differs from that of anti-influenza A antibodies, which are primarily directed towards epitopes on the virus surface glycoproteins. This finding may be relevant for the role of influenza A virus-specific CTL in recurrent infections with different influenza A virus.