Novel multi-photon microscopy based on resonant nonlinear optics of colloidal quantum dots
Novel multi-photon microscopy based on resonant nonlinear optics of colloidal quantum dots
复制标题
基于胶体量子点共振非线性光学的新型多光子显微镜
DOI：
10.1002/pssc.200880667
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Masia F
中科院分区：
文献类型：
--
作者：
Masia F
我们展示了一种基于探测胶体量子点发射的共振四波混频的新型成像模式。这种三阶信号是通过电子跃迁在可见光波长上被激发和检测的，这使得相对于双光子荧光显微镜的空间分辨率有了显著的提高。特别是，我们研究了由照明(光漂白)引起的样品的瞬时减少吸收随曝光时间和激发强度的依赖关系以及它对四波混频信号的影响(©2009 Wiley-VCH Verlag GmbH&Co.KGaA，Weinheim)
We demonstrate a novel imaging modality based on the detection of resonant four‐wave mixing emitted by colloidal quantum dots. This third‐order signal is excited and detected at a visible wavelength in resonance with an electronic transition, and results in a significant improvement of the spatial resolution with respect to a twophoton fluorescence microscope.In particular, we have investigated the dependence of the transient reduced absorption of the sample induced by the illumination (photobleaching) on the exposure time and excitation intensity, and its influence to the fourwave mixing signal (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)