Exosomes as Biomarker Treasure Chests for Prostate Cancer
Exosomes as Biomarker Treasure Chests for Prostate Cancer
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DOI：
10.1016/j.eururo.2010.12.031
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发表时间：
2011-05-01
期刊：
影响因子：
23.4
通讯作者：
Jenster, Guido
中科院分区：
文献类型：
--
作者：
Duijvesz, Diederick;Luider, Theo;Jenster, Guido
内容：尽管在标记物类型（蛋白质、DNA、RNA和代谢物）和改进技术（质谱、阵列和深度测序）的实施方面取得了进展，但在复杂流体（如血清和尿液）中发现前列腺癌（PCa）的新型生物标记物仍然是一个挑战。同时，最近的研究报道，许多癌症衍生的蛋白质和RNA分泌通过小囊泡称为exosomes.Objective：本文叙述性综述了exosomes研究的最新进展，重点是外泌体作为新的生物标志物PCa的潜在作用。本综述的目的是使泌尿外科领域的临床医生和研究人员了解外泌体作为生物标志物宝库的潜在作用及其临床价值。证据采集：使用关键词外泌体、微泡、前列腺体、生物标志物、前列腺癌和泌尿外科检索1966年至2010年9月的Medline和Embase条目。主要的出版物和文章建设性地有助于exosome research被选为this review.Evidence synthesis：exosome是小泡（50-100 nm）分泌的几乎所有组织;他们代表他们的组织起源。前列腺和PCa衍生的外泌体的纯化将使我们能够分析外泌体，为PCa提供有希望的蛋白质和RNA生物标志物来源。这种分析将有助于发现新的标志物的早期诊断和可靠的预后PCa.Conclusions：虽然初步结果是有希望的，需要进一步的调查，以评估这些外泌体在PCa的临床价值。(C)2010年欧洲泌尿外科协会。Elsevier B. V.出版，保留所有权利。
Context: Although progress has been made with regard to types of markers (protein, DNA, RNA, and metabolites) and implementation of improved technologies (mass spectrometry, arrays, and deep sequencing), the discovery of novel biomarkers for prostate cancer (PCa) in complex fluids, such as serum and urine, remains a challenge. Meanwhile, recent studies have reported that many cancer-derived proteins and RNAs are secreted through small vesicles known as exosomes.Objective: This narrative review describes recent progress in exosome research, focusing on the potential role of exosomes as novel biomarkers for PCa. The purpose of this review is to acquaint clinicians and researchers in the field of urology with the potential role of exosomes as biomarker treasure chests and with their clinical value.Evidence acquisition: Medline and Embase entries between 1966 and September 2010 were searched using the keywords exosomes, microvesicles, prostasomes, biomarkers, prostate cancer, and urology. Leading publications and articles constructively contributing to exosome research were selected for this review.Evidence synthesis: Exosomes are small vesicles (50-100 nm) secreted by almost all tissues; they represent their tissue origin. Purification of prostate- and PCa-derived exosomes will allow us to profile exosomes, providing a promising source of protein and RNA biomarkers for PCa. This profiling will contribute to the discovery of novel markers for the early diagnosis and reliable prognosis of PCa.Conclusions: Although the initial results are promising, further investigations are required to assess the clinical value of these exosomes in PCa. (C) 2010 European Association of Urology. Published by Elsevier B.V. All rights reserved.