Lipovitellin is a Non-Self Recognition Receptor with Opsonic Activity
Lipovitellin is a Non-Self Recognition Receptor with Opsonic Activity
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DOI：
10.1007/s10126-010-9315-x
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发表时间：
2011-06
影响因子：
3
通讯作者：
Jun Zhang;Shicui Zhang
中科院分区：
文献类型：
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作者：
Jun Zhang;Shicui Zhang
卵黄素（Lv）是一种糖脂蛋白，是蛋黄的主要成分，通常被认为是生长发育必需的营养物质的能量储备。我们通过两步色谱法从红倒刺Puntius conchonius的排卵卵中纯化了Lv，并通过高碘酸/希夫试剂和苏丹黑B染色、氨基酸组成分析和肽质量指纹图谱对其进行了表征。配体和细菌结合试验、酶联免疫吸附试验 (ELISA) 和吞噬试验的结果首次表明，纯化的天然形式的 P. conchoniusLv 是一种具有多种特异性的模式识别分子，能够识别病原体相关分子模式（PAMP），包括脂多糖、脂磷壁酸和肽聚糖，而不是自身成分，并且它可以结合革兰氏阴性和阳性细菌，例如大肠杆菌和金黄色葡萄球菌。这些测试还表明，P. conchoniusLv 作为调理素发挥作用，能够增强巨噬细胞的吞噬作用。总而言之，这些特征表明在发育中的P.在 conchonius 中，Lv 的天然形式可能在生理上参与通过与 PAMP 相互作用感知入侵的病原体，并参与招募早期胚胎中出现的原始巨噬细胞来吞噬和消化病原体，从而保护它们免受病原体攻击。
Lipovitellin (Lv), a glycolipoprotein, is a major component of the egg yolk, which is usually regarded as an energy reserve of nutrients essential for growth and development. We have purified Lv from ovulated eggs of the rosy barbPuntius conchoniusby two-step chromatography and characterized it by staining with periodic acid/Schiff reagent and Sudan black B, amino acid composition analysis, and peptide mass fingerprinting. The results of ligand and bacterial binding assays, an enzyme-linked immunosorbent assay (ELISA), and the phagocytosis test revealed, for the first time, that the purified native form ofP. conchoniusLv acts as a pattern recognition molecule with multiple specificities capable of identifying pathogen-associated molecular patterns (PAMPs), including those of lipopolysaccharide, lipoteichoic acid, and peptidoglycan, rather than self components and that it can bind Gram-negative and -positive bacteria, such asEscherichia coliandStaphylococcus aureus. These tests also showed that theP. conchoniusLv functions as an opsonin capable of enhancing macrophage phagocytosis. Taken together, these characteristics suggest that in developing embryos/larvae ofP. conchonius, the native form of Lv may be physiologically involved in the sensing of invading pathogens via interaction with PAMPs and in the recruitment of the primitive macrophages that appear in early embryos to phagocytose and digest the pathogens, thereby protecting them from pathogenic attacks.