Role of novel polysaccharide layers in assembly of the exosporium, the outermost protein layer of the Bacillus anthracis spore.
Role of novel polysaccharide layers in assembly of the exosporium, the outermost protein layer of the Bacillus anthracis spore.
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新型多糖层在炭疽芽孢杆菌孢子外孢子层（炭疽芽孢杆菌孢子最外层蛋白质层）组装中的作用。
DOI：
10.1111/mmi.14966
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发表时间：
2022-09
影响因子：
3.6
通讯作者：
Driks, Adam
中科院分区：
文献类型：
--
作者：
Lehmann, Dorte;Sladek, Margaret;Khemmani, Mark;Boone, Tyler J.;Rees, Eric;Driks, Adam
细胞生物学中的一个基本问题是细胞如何组装它们的外层。细菌内孢子是细胞层组装的成熟模型。然而，外孢子菌的组装仍然知之甚少。外孢子菌是一种复杂的蛋白质壳，包括病原体炭疽杆菌的最外层。外孢子菌的组装始于孢子表面一侧的蛋白质沉积，然后逐渐包围孢子。我们试图解决一个重大的悬而未决的问题：将外孢子菌组装到孢子上，然后进入闭合壳的机制。我们假设，外孢子体(间隙)正下方的物质将外孢子体组装到孢子上，并驱动包围。为了支持这一点，我们证明了空隙至少有两层不同的多糖层。其次，我们证明了外孢子菌的包围需要可能的多糖生物合成基因，这表明了间隙的直接作用。这些结果不仅显著阐明了外孢子菌(一种分布特别广泛的细菌外层)的组装机制，而且提出了一种新的机制，即多糖层驱动蛋白质壳的组装。在许多芽孢杆菌孢子中的蛋白质外壳外层--外孢子体的组装中，多糖起到了以前未知的作用。外孢子菌的组装始于初始层(黄色)的沉积，由多糖(红色)(A)连接到发育中的孢子表面的一侧。外孢子菌(绿色)继续聚集在孢子周围，最终包围它(C，D)，同时积累第二层多糖层(蓝色)。
A fundamental question in cell biology is how cells assemble their outer layers. The bacterial endospore is a well-established model for cell layer assembly. However, the assembly of the exosporium, a complex protein shell comprising the outermost layer in the pathogen Bacillus anthracis, remains poorly understood. Exosporium assembly begins with deposition of proteins at one side of the spore surface, followed by the progressive encirclement of the spore. We seek to resolve a major open question: the mechanism directing exosporium assembly to the spore, and then into a closed shell. We hypothesized that material directly underneath the exosporium (the interspace) directs exosporium assembly to the spore and drives encirclement. In support of this, we show that the interspace possesses at least two distinct layers of polysaccharide. Secondly, we show that putative polysaccharide biosynthetic genes are required for exosporium encirclement, suggesting a direct role for the interspace. These results not only significantly clarify the mechanism of assembly of the exosporium, an especially widespread bacterial outer layer, but also suggest a novel mechanism in which polysaccharide layers drive the assembly of a protein shell. Polysaccharide has a previously unknown role in assembly of the exosporium, a protein-shell outer-layer in many Bacillus spores. Exosporium assembly begins with deposition of an initial layer (yellow), connected to one side of the developing spore surface by polysaccharide (red) (A). Exosporium (green) continues to assemble around the spore, ultimately encircling it (C, D), while a second polysaccharide layer (blue) accumulates.
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影响因子：
4
作者：
Bojar, Daniel;Meche, Lawrence;Meng, Guanmin;Eng, William;Smith, David F.;Cummings, Richard D.;Mahal, Lara K.
通讯作者：
Mahal, Lara K.
影响因子：
9.9
作者：
Arrieta-Ortiz ML;Hafemeister C;Bate AR;Chu T;Greenfield A;Shuster B;Barry SN;Gallitto M;Liu B;Kacmarczyk T;Santoriello F;Chen J;Rodrigues CD;Sato T;Rudner DZ;Driks A;Bonneau R;Eichenberger P
通讯作者：
Eichenberger P
影响因子：
2.7
作者：
Beckmann HS;Möller HM;Wittmann V
通讯作者：
Wittmann V
影响因子：
4.8
作者：
Daubenspeck, JM;Zeng, HD;Turnbough, CL
通讯作者：
Turnbough, CL
影响因子：
3.8
作者：
Bozue, JA;Parthasarathy, N;Friedlander, AM
通讯作者：
Friedlander, AM