RNA-binding specificity of Y-box protein 1.
RNA-binding specificity of Y-box protein 1.
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DOI：
10.4161/rna.6.1.7458
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发表时间：
2009-01
期刊：
影响因子：
4.1
通讯作者：
Meric-Bernstam F
中科院分区：
文献类型：
--
作者：
Dong J;Akcakanat A;Stivers DN;Zhang J;Kim D;Meric-Bernstam F
Y-box protein 1 (YB-1) is a multifunctional DNA/RNA-binding protein that regulates transcription and translation. The specificity of YB-1’s RNA binding and its consequences are unknown. Because expression and subcellular localization of YB-1 have been reported to be important in breast cancer, we determined the specificity and functional impact of YB-1 mRNA-binding in MCF7 breast cancer cells. We used YB-1 antibodies to immunoprecipitate YB-1 and microarray profiling to compare YB-1-bound and total poly(A) RNA. We demonstrated that YB-1 mRNA-binding was preferential. Transcript sequences significantly associated with this binding had high GC content. Selected YB-1 mRNA-binding targets were confirmed by QRT-PCR. However, downregulation of YB-1 levels by siRNA did not affect their RNA or protein expression. Thus, YB-1 has RNA-binding specificity; however, YB-1 binding does not necessarily regulate the stability or translation of its mRNA targets. Further study is needed to determine the functional consequences of selective YB-1 mRNA binding.
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