Effects of cannabinoid (CBD) on blood brain barrier permeability after brain injury in rats
Effects of cannabinoid (CBD) on blood brain barrier permeability after brain injury in rats
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DOI：
10.1016/j.brainres.2021.147586
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发表时间：
2021-07-28
期刊：
影响因子：
2.9
通讯作者：
Li, Ping
中科院分区：
文献类型：
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作者：
Jiang, Hongyan;Li, Hengxi;Li, Ping
大麻二酚是一种天然草药，已知可保护大脑免受创伤性脑损伤（TBI）。在此，建立TBI大鼠模型，在手术前30分钟和手术后6小时直至处死，以5、10或20 mg/kg的剂量腹膜内施用大麻二酚。测定脑含水量、体重和改良神经系统严重程度评分，并进行酶联免疫吸附测定、免疫荧光染色、苏木精和伊红染色、尼氏染色、伊文思蓝染料外渗和蛋白质印迹。结果表明，大麻二酚减少了水通道蛋白4阳性和胶质纤维酸性蛋白阳性细胞的数量。大麻二酚还显著降低了促炎细胞因子（TNF-α和IL-1 β）的蛋白水平，并显著增加了紧密连接蛋白（claudin-5和occludin）的表达。此外，大麻二酚给药显着减轻了TBI和血脑屏障破坏后大脑中的含水量，并改善了TBI后的神经功能缺损评分。大麻二酚给药改善了血脑屏障的完整性和渗透性，并减少了TBI后脑水肿。
Cannabidiol is a natural herbal medicine known to protect the brain from traumatic brain injury (TBI). Here, a TBI rat model was established, with cannabidiol administered intraperitoneally at doses of 5, 10, or 20 mg/kg, 30 min before surgery and 6 h after surgery until sacrifice. Brain water content, body weight, and modified neurological severity scores were determined, and enzyme-linked immunosorbent assay, immunofluorescence staining, hematoxylin and eosin staining, Nissl staining, Evans-blue dye extravasation, and western blotting were performed. Results showed that cannabidiol decreased the number of aquaporin-4-positive and glial fibrillary acidic protein-positive cells. Cannabidiol also significantly reduced the protein levels of proinflammatory cytokines (TNF-alpha and IL-1 beta) and significantly increased the expression of tight junction proteins (claudin-5 and occludin). Moreover, cannabidiol administration significantly mitigated water content in the brain after TBI and blood-brain barrier disruption and ameliorated the neurological deficit score after TBI. Cannabidiol administration improved the integrity and permeability of the blood-brain barrier and reduced edema in the brain after TBI.