The stereotyped positioning of the generative cell associated with vacuole dynamics is not required for male gametogenesis in rice pollen
The stereotyped positioning of the generative cell associated with vacuole dynamics is not required for male gametogenesis in rice pollen
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水稻花粉雄配子发生不需要与液泡动力学相关的生殖细胞的定型定位
DOI：
10.1111/nph.15038
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发表时间：
2018-04-01
期刊：
影响因子：
9.4
通讯作者：
Sun, Meng-Xiang
中科院分区：
文献类型：
--
作者：
Huang, Xiaorong;Peng, Xiongbo;Sun, Meng-Xiang
在谷物中的雄性配子发生期间，生殖细胞经历与中央液泡的动力学平行的定位过程，这被认为与雄性配子体中的生殖细胞运动有关。然而，生殖细胞定位和中央液泡动态对雄性配子发生的影响仍然知之甚少。在这里，我们报告OsGCD 1（GAMETE CELLS DEFECTIVE1）功能障碍影响花粉发育和破坏花粉萌发。OsGCD 1功能的丧失改变了中央空泡的动力学，生殖细胞被错误定位。然而，双精子细胞正常产生，表明配子发生不依赖于位置信息，只要产生生殖细胞。正常的液泡动态似乎仅对花粉成熟和萌发是必需的。我们的研究结果还表明，osgcd1突变导致水稻雄性不育，其中花粉具有完整的细胞活力，并产生正常的配子，但缺乏发芽的潜力。
During male gametogenesis in cereals, the generative cell undergoes a positioning process that parallels the dynamics of the central vacuole, which is believed to be associated with generative cell movement in the male gametophyte. However, the impact of the generative cell positioning and the central vacuole dynamics on male gametogenesis has remained poorly understood. Here, we report that OsGCD1 (GAMETE CELLS DEFECTIVE1) dysfunction influenced pollen development and disrupted pollen germination. Loss of function of OsGCD1 altered the central vacuole dynamics and the generative cell was mispositioned. Nevertheless, twin sperm cells were generated normally, indicating that gametogenesis does not rely on positional information as long as a generative cell is produced. The normal vacuole dynamics seems necessary only for pollen maturation and germination. Our findings also indicate that osgcd1 mutation resulted in rice male sterility in which pollen has full cell viability and generated normal gametes, but lacks the potential to germinate.