Origin of excitation underlying locomotion in the spinal circuit of zebrafish
Origin of excitation underlying locomotion in the spinal circuit of zebrafish
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DOI：
10.1073/pnas.1115377109
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发表时间：
2012-04-03
影响因子：
11.1
通讯作者：
El Manira, Abdeljabbar
中科院分区：
文献类型：
--
作者：
Eklof-Ljunggren, Emma;Haupt, Sabine;El Manira, Abdeljabbar
脊髓中的神经回路将来自大脑的指导性信号转换成协调良好的运动，这是通过产生节奏的组件实现的。虽然有证据表明兴奋性中间神经元是运动节律产生的本质，但它们的分子身份及其必要性的评估仍不清楚。在这里，我们表明，使用幼体斑马鱼，V2A中间神经元代表的运动节奏的正常表达所需的内在来源的兴奋。急性和选择性消融这些interneurons增加诱导游泳活动的阈值，降低突发频率，并改变活动的喙尾传播的协调。因此，我们的研究结果表明，V2a中间神经元代表了一个兴奋源，赋予脊髓回路产生运动的能力。
Neural circuits in the spinal cord transform instructive signals from the brain into well-coordinated locomotor movements by virtue of rhythm-generating components. Although evidence suggests that excitatory interneurons are the essence of locomotor rhythm generation, their molecular identity and the assessment of their necessity have remained unclear. Here we show, using larval zebrafish, that V2a interneurons represent an intrinsic source of excitation necessary for the normal expression of the locomotor rhythm. Acute and selective ablation of these interneurons increases the threshold of induction of swimming activity, decreases the burst frequency, and alters the coordination of the rostro-caudal propagation of activity. Thus, our results argue that V2a interneurons represent a source of excitation that endows the spinal circuit with the capacity to generate locomotion.