Heme oxygenase-1 (HO-1) is constitutively up-regulated in top alpinists.
Heme oxygenase-1 (HO-1) is constitutively up-regulated in top alpinists.
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DOI：
10.1016/j.bbrc.2011.11.064
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发表时间：
2012-01
影响因子：
3.1
通讯作者：
Gota Miura;Kazunori Kato;Takahiko Shimizu;D. Shiga;T. Shirasawa
中科院分区：
文献类型：
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作者：
Gota Miura;Kazunori Kato;Takahiko Shimizu;D. Shiga;T. Shirasawa
挑战珠穆朗玛峰的登山运动员在珠穆朗玛峰进攻前需要适应缺氧。尽管这种适应对于成功攀登珠穆朗玛峰很重要，但适应缺氧的分子机制尚不清楚。为了阐明这一机制，我们利用微阵列分析研究了顶级登山运动员特有的缺氧诱导基因表达。我们在此报告，与非登山运动员相比，顶尖登山运动员血液中的血红素加氧酶-1 (HO-1) 显着升高。尽管非登山者的 HO-1 表达也会因缺氧而上调，但顶尖登山者的 HO-1 水平在结构上高于非登山者。对一名顶级登山运动员HO-1的系列检查显示，在顶峰袭击后几个月，HO-1的较高表达仍维持在高水平。结合 HO-1 催化血红素转化为 CO 和胆红素的生化功能，HO-1 的表达可以改善循环并补偿缺氧引起的氧化组织损伤。这些数据还表明，顶级登山运动员的外周血通过维持高水平的HO-1表达而具有不依赖于抗原的缺氧记忆，这有助于快速适应8000m攀登的缺氧。
Alpinists who challenge Mt. Everest need adaptation to hypoxia before the attack of Mt. Everest. Although this adaptation is important for the success of climbing Mt. Everest, the molecular mechanism on the adaptation to hypoxia is not well understood. In order to clarify this mechanism, we investigated hypoxia-induced gene expressions specific for top alpinists using microarray analyses. We report here that heme oxygenase-1 (HO-1) is significantly higher in the blood of top alpinist compared with non-alpinists. Although HO-1 expression of non-alpinists is also up-regulated in response to hypoxia, HO-1 level of the top alpinists are constitutively higher than that of non-alpinists. Serial examinations of HO-1 in one top alpinist revealed that the higher expression of HO-1 is maintained in high-level several months after the attack of top mountains. Taken together with the biochemical function of HO-1 that catalyzes heme into CO and bilirubin, HO-1 expression may improve the circulation and compensate with oxidative tissue damages induced by hypoxia. These data also suggest that peripheral blood has the memory on hypoxia independent of antigens by maintaining the high-level of HO-1 expression in top alpinists, which merits the rapid adaptation to hypoxia for 8000m climbing.